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细菌L型败血症的诊断和治疗
本文报告细菌L型败血症30例.细菌学证明金黄色葡萄球菌28例,变形杆菌和四联球菌各1例.单独L型菌12例,L型伴细菌型18例.细菌L型是细菌发生变异后细菌细胞壁的缺陷型.它不宜在常规培养基生长,能在高渗L型培养基中生长繁殖.若加用L型培养基,血培养阳性率可由18.4%提高到61.5%.临床L型菌多属不稳定型菌株,易于返祖成细菌型.细菌L型败血症有以下特点:(1)发热在疗程中起伏不易控制;(2)自细胞总数常不升高,但有核左移、中性粒细胞胞浆空泡、中毒颗粒等中毒退行变;(3)伴发问质性肺炎,发生率86.7%,可作为诊断线索;(4)部分患者迁延难愈;(5)血培养前半数用过青霉素G,所得L型菌对作用于细胞质抗生素较敏感.
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蛋白酶活性检定及其在特定基质1D与2D凝胶中的特性:一种生物样品中分离、鉴定新的活性型蛋白酶的方法
The emergence of bacterial pathogen resistance to common antibiotics strongly supports the necessity to develop alternative mechanisms for combating drug-resistant forms of these infective organisms. Currently, few pharmaceutical companies have attempted to investigate the possibility of interrupting metabolic pathways other than those that are known to be involved in cell wall biosynthesis. Bacterial proteases have been showed to play an important role during infection and their inhibitors can retard the growth, proliferation and invasion of bacterial pathogens. To separate and identify these proteases, we have developed a specific, sensitive assay in SDS-polyacrylamide gels after 2D electrophoresis. This method allows simultaneous determination of protease cleavage specificity, molecular weight, isoelectric point, and if necessary, amino acid sequencing.
