重组腺相关病毒介导CD151基因治疗心肌梗死后侧支循环形成

目的 探讨重组腺相关病毒(rAAV)介导的CD151基因转染对小型猪心肌梗死模型后侧支循环重建的影响.方法 中国实验用小型猪(清洁级,由华巾农业大学种猪场提供)26头,随机分为:(1)正常对照绀(n=4),健康小型猪,小进行任何手术处理;(2)rAAV-GFP组(n=7),结扎冠状动脉序前降支(left anterior descending,LAD)制备小型猪急性心肌梗死模型,心电监护显示I、aVL导联ST段持续抬高20 min为模型制备成功,同时,在左心室前壁梗死边缘区心肌分10点注射rAAV-GFP(1×10 11 pfu/点);(3)rAAV-CD151纽(n=7),结扎LAD,左窜前壁梗死边缘区心肌分10点注射rAAV-CD151;(4)rAAV-anti-CD151组(n=8),结扎LAD,左室前壁梗死边缘区心肌分10点注射rAAV-anti-CD151.8周后用Western Blot测定心肌组织CD151蛋白的表达,冠脉造影评价冠脉侧支循环形成情况.氯化二苯基四氮唑(TTC)染色显示心肌梗死面积.运用单因素方差分析进行统计学分析,以P＜0.05为差异具有统汁学意义.结果 与正常对照组和rAAV-GFP组比较,rAAV-CD151组心肌组织局部CD151蛋白表达增加(P＜0.05).冠状动脉造影证实CD151基因转染明显促进闭塞动脉的侧支循环建立.rAAV-CD151组心肌梗死面积百分比明显小丁rAAV-GFP组[(12.82±2.26)%vs.(23.14±2.83)%,P＜0.05],rAAV-anti-CD151纽心肌梗死面积百分比(32.52±3.47)%明显大于rAAV-GFP组和rAAV-CD151组(P＜0.05).结论 CD151基因转染可以明显促进小型猪急性心肌梗死后侧支循环的建立,减小心肌梗死面积.

Objective To evaluate the use of the saphenous artery as an alternative access for endovascular procedures in the porcine model. Methods Fourteen adult pigs (25-35 kg) were used in this study, 3 pigs from an acute study and 11 from chronic studies. A 2-3 cm incision was made and a saphenous artery cutdown was performed in 24 sides. Micropuncture sets (Boston Scientific) or 18 G puncture needles were used to access the artery. Different sizes of introducers (4-7 F) were used to establish endovascular access. Angiographic catheters were then used to confirm if the access was usable. Four saphenous arteries were explanted in the pigs from a chronic study 4 to 28 days after surgical procedure. Results The saphenous artery was very easy to expose and 4-5 F introducer sheaths were able to be inserted to establish access for endovascular procedures in the pigs. The saphenous artery was unable to accomodate an introducer with a size larger than 6 F. Four saphenous arteries were injured when 5 and 6 F introducers were used, but angiographic procedures could still be performed. Morphologic evaluation of the explanted arteries demonstrated occlusion of the saphenous arteries without injury or disruption of the adjacent femoral arteries. Conclusion The saphenous artery can be used as an access site in pigs for angiographic and interventional procedures if the catheter size is less than 6 F. This vessel is easier to access and can preserve the femoral artery for repeat procedures in the future.

Objective: TO verify through animal experiment the validity of chemical cholecystectomy . Methods: The experimental objects seven healthy juvenile pigs,hardener was infused into the gallbladder,after infusion the samples were collected by pathoiogical examination , according to the different duration under anesthestize. Reslts:The mucous destructive and digestive process remained with one week, the inflammatory reacton in two weeks,the chronic inflatoy reaction compained a a great deal of granu lation tissue and scar formation occurred in 4th-8th week,10 weeks latter,the inflmmatory reaction reduced ,and scar tissue formed. Conclusion: Chemical cholecystectomy is safe and reliable in clinical.

Objective:To verify through animal experiment the validity of chemical cholecystectomy.Mothods:The expermental objects seven healthy juvenile pigs,hardener was infused into the gallbladder,after infusion the sapmles were collected by pathological examination,according to the different duration under anesthestize.Results:The mucous destructive and digestive process remained with one week,the inflammatory reaction in two weeks,the chronic inflammatory reaction compained a great deal of granulation tissue and scar formation occurred in 4th-8th week,10 weeks latter,the inflammatory reaction reduced,and scar tissue formed.Conlusion:Chemical cholecystecomy is safe and reliable in clinic.