欢迎来到360期刊网!
学术期刊
  • 学术期刊
  • 文献
  • 百科
电话
您当前的位置:

首页 > 学术期刊 > 儿科学 > 中华儿科杂志

中华儿科

中华儿科杂志

Chinese Journal of Pediatrics 중화아과잡지

北大核心期刊
  • 主管单位: 中国科学技术协会
  • 主办单位: 中华医学会
  • 影响因子: 2.31
  • 审稿时间: 1-3个月
  • 国际刊号: 0578-1310
  • 国内刊号: 11-2140/R
  • 发行周期: 月刊
  • 邮发: http://www.cmaped.org.cn/
  • 曾用名:
  • 创刊时间: 1950
  • 语言: 英文
  • 编辑单位: 中华儿科杂志编辑委员会
  • 出版地区: 北京
  • 主编: 杨锡强
  • 类 别: 儿科学
期刊荣誉:
  • 北京地区人群中人Boca病毒血清抗体的分析

    作者:赵林清;钱渊;朱汝南;邓洁;王芳;董慧瑾

    Objective To find out the importance of human bocavirus (HBoV) as an infectious agent for population in Beijing, China. Seroprevalence study was conducted by using expressed recombinant major capsid VP2 protein as an antigen.Methods Serum specimens collected from infants and children who visited the Children's Hospital Affiliated to the Capital Institute of Pediatrics for health check-up and adults visiting the Xuanwu Hospital, Beijing for diseases other than respiratory infections from April 1996 to March 1997 were used for the investigation. The major capsid protein VP2 from HBoV was expressed in E. coli strain BL21 (DE3) with the transformed PET30b vector inserted with full-length VP2 gene of HBoV and the specific antigenicity of this expressed protein was validated by previous study. Western blotting was used to detect specific IgG antibody against HBoV in collected serum specimens diluted to 1:200. Mock expressed protein was E. coli cells strain BL21 (DE3) with the transformed PET30b vector without insert. Anti-His monoclonal antibody and rabbit anti-HBoV VP2 polypeptides hyper-immune serum were used as positive control for antibody detection.Results Out of 677 serum specimens tested, 400 (59.1% ) were positive for HBoV by Western blotting. About 45.3% (34/75) of the newborns under 1 month of age had anti-HBoV antibodies, and antibody positive rates were decreased in age groups of 1 and 2 months (41.4% and 31.3%, respectively) then increased in the following ages from 6 months to 7 years old ( from 45.6% to 69.7% ). The antibody positive rates were maintained at a relatively constant level ( about 70% ) in the age groups from 7 years to 40 years of age and became lower ( 61.8% - 62. 8% ) in those over 50 years.Conclusions The high seroprevalence of antibody against recombinant HBoV VP2 protein and early age antibody acquisition indicate that HBoV has been circulating in population of Beijing, China as early as in 1996 and most of children had been exposed to HBoV by the age of 7 years. Infants under the age of 6 months were susceptible to this virus.

  • 20例汉族新生儿呼吸窘迫综合征的SP-B遗传缺陷的研究

    作者:尹晓娟;罗分平;李爱华;安玉林;封志纯

    Objective To investigate the relationship between expression of surfactant protein B (SP-B) gene product and neonatal respiratory distress syndrome (NRDS) in Han ethnic group.Methods Unrelated 20 cases with NRDS of Han ethnic group were selected as NRDS group while unrelated 20 cases of Han ethnic group with other diseases were selected as a control. The cases in the control group had congenital heart disease or bronchopulmonary dysplasia or persistent pulmonary artery hypertension. Blood sample was taken from each case. Lung tissues were taken from the patients in half an hour after their death in the two groups. Expression of SP-B in the lung tissues was determined with immunohistochemical technique. Genetic deficiency variant of SP-B intron Ⅳ was screened with polymerase chain reaction (PCR).Results Two cases at gestational age of 26 weeks, one at 34 weeks and two at 42 weeks in the NRDS groups had lower expression level of SP-B in the lung tissues than those at the same age in the the control group. Expression of SP-B in the lung tissues of the control group increased with gestational age, but no such phenomenon was found in NRDS group. Further two cases at gestational age 42 weeks of NRDS group had genetic deficiency variant of SP-B intron Ⅳ with gene analysis of five cases who had lower expression of SP-B. Clinical data suggest that patients at 42 weeks of gestational age had severe illness.Conclusion Decrease of SP-B expression may be involved in occurrence of NRDS, genetic deficiency variant of SP-B intron Ⅳ exists in NRDS cases of Han ethnic group of China.

  • 肠道病毒71型感染首发肺水肿与肺出血三例报告

    作者:何时军;王霞;郑晓群;王传夏;黄爱蓉;金益梅;杨好妹;周爱华

    Objective To investigate the clinical features of the enterovirus 71 ( EV71 ) infection complicated with pulmonary edema or pulmonary hemorrhage as a fulminant and often fatal illness.Methods The medical records of three cases with EV71 infection were retrospectively reviewed for clinical manifestations, laboratory data, medications, and outcome.Results All the cases were infants and died of the infection. These infants had no skin or mucosal lesions, however, they had sudden onset of cyanosis and tachypnea 1 to 2 days after the onset of the febrile disease with vomiting. All these 3 cases were misdiagnosed and were treated for shock on admission. Pulmonary hemorrhage was not considered in any of the cases on admission. All the cases received tracheal intubation when foamy secretions were discharged from the mouth and nose of the patients and notable cyanosis occurred. After intubation, pink foamy fluid flew out from the endotracheal tube in all the 3 cases. The patients had hyperglycemia and limb weakness, two had tachycardia, and hypertension was found in one case. Chest X-ray showed bilateral or unilateral widespread air space opacity, but the cardiac size and shape were normal. All the patients had leukocytosis. Enterovirus 71 infection was confirmed by detection of specific nucleic acid sequences of the virus from throat swab and tracheal secretions samples and in one case in cerebrospinal fluid.Conclusions Pulmonary edema or pulmonary hemorrhage occurred in the 3 cases with EV71 infection. The initial presentation was nonspecific with fever and vomiting, and sudden appearance of cyanosis, tachypnea, tachycardia, hypertension or hypotension, limb weakness, which may suggest pulmonary edema or hemorrhage. Excessive fluid resuscitation may deteriorate the illness, on the contrary, fluid restriction and inotropic agents, and early intubation with positive pressure mechanical ventilation may be the proper treatment.

  • 特发性婴儿动脉钙化症一例

    作者:谢宗德;盖建芳;李雯

    SE REPORTA female infant, 30 min after birth, was transferred to neonatal intensive care unit (NICU) from the Department of Obstetrics on October 7th, 2006 because of anasarca and no spontaneous breath. The infant was born to her mother at gravida 3, para 3, and her gestational age was 34 weeks. She was delivered by cesarean section because of intrauterine distress and polyhydramnios. Prenatal color Doppler flow imaging ( CDFI ) suggested bilateral hydronephrosis and ascites. The Apgar scores were 3 at 1 min and 7 at 5 min. Amniotic fluid was clear but excessive. The infant had anasarca, cyanosis and had no spontaneous breath at birth. She was admitted to the NICU after tracheal intubation.

  • 推动高质量的临床研究实现对儿童健康的承诺

    作者:桂永浩

    Child health care in the 21st Century faces many new problems and challenges. By the year 2015, the mortality rate of children under the age of 5 years should be reduced by two thirds and the maternal mortality by three fourth[1], as proposed in the Millennium Development Goals by the World Health Organization and the United Nations Children's Fund. China is a large developing country with more than 300 million children. According to Outline of Health Care Development of the 11th Five-year Plan[2] , by 2010, China's infant mortality rate must be lowered to 14.9‰, the mortality rate of children under 5 years of age to 17.7‰, and maternal mortality under 0. 0004%. The realization of these objectives will be a comprehensive reflection of the child health policies of the 21st century in lowering child mortality rate, protecting children, and facilitating their development, as well as safeguarding and promoting children's health.

  • 我国儿科临床科学研究的现状与思考

    作者:杨锡强

    Various definitions have been given to the term "scientific research". It is described as " pursuing truth and exploring knowledge", " critical thinking to develop knowledge", or " collection, synthesis, and analysis of information" . In China, the Ministry of Education proposed that scientific research is to "promote and apply knowledge to invent new techniques and provide creative work". Based on the definitions given above, we describe scientific research as " to explore what we do not know, and to collect, analyze, distinguish, apply, and evaluate what we have known". It is characterized by innovation and non-replication, which involve discontinued and complicated exploration. According to this definition, medical scientific research is therefore defined as investigating the nature of human life and rules of illness, as well as seeking methods to prevent and treat diseases, and improve people's health.

  • 儿童缺铁和缺铁性贫血防治建议

    作者:《中华儿科杂志》编辑委员会;中华医学会儿科学分会血液学组;中华医学会儿科学分会儿童保健学组;高举

    INTRODUCTIONIron deficiency, the most common nutrient deficiency, has been a global health problem, affecting one third of the world population. The condition is especially severe in developing countries. According to the data from WHO, the prevalence rates of anemia in these areas were 48% and 39% respectively in children under 5 years of age and between 5 - 14 years, of whom, over half cases were iron deficiency anemia (IDA). Furthermore, the prevalence rate of iron deficiency is at least 2 times that of IDA. Thanks to some well-organized public health education and widespread food iron fortification programs, the prevalence rate of IDA has declined remarkably during the last three decades. According to the US National Health and Nutrition Examination Survey (NHANES 1999-2000) , the rates of iron deficiency and IDA in children aged 1-2 years were 7% and 2% respectively (however, the rate of iron deficiency was still up to 17% in Hispanic descendents).

  • 重视儿童缺铁性贫血的防治

    作者:李俊;黎海芪

    Deficiencies of iron, vitamin A, and iodine are the three most common nutritional diseases affecting children's health around the world. Deficiencies of vitamin A and iodine can be prevented through dietary adjustment or fortification strategies. However, the treatment for iron deficiency (ID) is complex, since it might be caused by a number of factors that decrease the biological activity and absorption of iron; and the iron status of the body can not be evaluated simply by iron intake. Patients with ID can develop anemia in weeks or months when the iron stores are depleted.

  • 神经源性肺水肿

    作者:孙若鹏;赵翠芬

    Neurogenic pulmonary edema (NPE) is a type of pulmonary edema that occurs secondary to central nervous sytem (CNS) damage, namely centrogenic pulmonary edema or cerebrogenic pulmonary edema[1,2] NPE is clinically characterized by acute dyspnea and progressive hypoxemia, while tachycardia, hypertension and tachypnea are only nonspecific symptoms in early phase. Early diagnosis of NPE is difficult since chest X-ray shows no remarkable sign or only increased hazy lung markings in early stage[3]. Diagnosis can be made definitely in the late stage of NPE according to the following manifestation : paleness, clamminess, feeling of impending death, rales, frothy pink sputum, hypoxemia and bilateral widespread infiltration on chest roentgenography. However, successful rescue rate is very low and mortality rate could reach as high as 90% at this stage[4-6].

  • 免疫毒素2E8-去甲斑蝥素的研制及其体外靶向抑制效应研究

    作者:李丽霞;汤永民;张海忠;沈红强;钱柏芹;罗春芳

    Objective The immunotoxins generated by conjugating monoclonal antibody (mAb) and a certain toxin play an important and promising role in treating hematopoietic malignancies. However, most of the toxins used for the conjugation are toxic proteins, which are immunogenic in the patients. Norcantharidin (NCTD) is a small molecule toxin without immunogenicity, and thus has become a potential new drug for hematopoietic cancers. In this study, we prepared immunotoxin 2E8-NCTD by using the ZCH-4-2E8 cells produced in the laboratory of our hospital, and then detected its targeting effect against CD+19 lymphoid malignant Nalm-6 cells in vitro.Methods 2E8 mAb was obtained from mouse ascites and purified by gel chromatography. After its purity was checked by SDS-PAGE, immunotoxin 2E8-NCTD was generated by conjugating CD19 mAb with NCTD using activated ester method. The binding activity of the immunoconjugate to CD19 antigens on cell surface, and the expression levels of the CD19 antigens on Nalm-6 and K562 cells were determined by flow cytometry. The inhibitory effects of PBS, purified 2E8 mAb, NCTD, and immunotoxin 2E8-NCTD on the cell growth of either Nalm-6 or K562 cells were then compared.Results The purity of the 2E8 mAb was higher than 99% demonstrated by SDS-PAGE assay. 2E8 mAb was detected on the surface of 99.34% of the Nalm-6 cells, while on only 0.98% of the K562. The newly generated immunotoxin had a positive rate of 99.90% on the Nalm-6 with slightly reduced binding activity. Both 2E8-NCTD and NCTD significantly inhibited the growth of CD+19 Nalm-6 cells (P < 0. 001 ), while the purified 2E8 mAb did not show any significant influences on the growth of the same cell line ( P > 0.05 ). Meanwhile, no significant inhibitory effects on the CD-19 K562 cells were identified in the 2E8-NCTD, 2E8 mAb, or control groups, indicating a significant targeting effect of 2E8-NCTD against Nalm-6 cells.Conclusions The immunotoxin 2E8-NCTD can be synthesized by activated ester method. It has target killing effects on CD+19 Nalm-6 leukemia cells in vitro.

  • 儿童急性T淋巴细胞白血病T细胞受体β链基因重排的特点及其在微小残留病定量检测中的意义

    作者:刘婕好;李志刚;高超;崔蕾;吴敏媛

    Objective To explore the characteristics of T-cell receptor beta (TCRβ) gene rearrangement in children with T-cell acute lymphoblastic leukemia (T-ALL) and establish a system for quantitative detection of minimal residual disease (MRD) by real-time quantitative PCR (RQ-PCR) targeting the TCRβ gene rearrangement. Methods Multiplex PCR designed by the BIOMED-2 was used to detect TCRβ gene rearrangement in the bone marrow samples of 26 children with T-ALL. Sequences of junctional region were then compared and analyzed in IMGT database. Allele specific oligonucleotide (ASO) upstream primers were designed complementary to the V-D-J or D-J junctional region of TCRβ gene rearrangements. Samples at diagnosis were serially diluted in DNA obtained from mononuclear cells (MNC) from a pool of 20 healthy donors to generate the patient-specific standard curves. Subsequently, TCRβ RQ-PCR was applied to six patients to quantify MRD with germline Jβ primer/probe combinations. To determine the quantity and quality of DNA, we also used RQ-PCR for the N-ras gene.Results Clonal rearrangements were identified in 92.3% of the children with T-ALL ( Vβ-Dβ-Jβ rearrangements in 84.6% and Dβ-Jβ rearrangements in 50% ). Comparative sequence analysis of 42 TCRβ recombination revealed that two downstream Vβ families (BV5, BV6) were preferentially used. The segment Jβ2. 7 was dominant in childhood T-ALL. Jβ1. 3, Jβ2.4, and Jβ2.6 were not detected. The slope of the standard curves was from - 3.54 to -3.37 with the intercepts between 19.35 and 20.51. The correlation coefficients of all the 6 standard curves were ≥0.98. None of the cases had a quantitative range of RQ-PCR lower than 10<'-4>. During the follow-up, an increased incidence of MRD was found before relapse.Conclusions RQ-PCR, which is a highly sensitive and specific method for detection of TCRβ gene rearrangements, will be of high value to study MRD in T-ALL. Close monitoring of MRD is of great importance for prognosis and follow-up of the patients with the disease.

  • 对"肠道病毒71型感染首发肺水肿与肺出血三例报告"一文的看法

    作者:陈贤楠

    Cases with severe enterovirus 71 ( EV71 ) infection have been reported outside the mainland of China since the 1990s[1]. The epidemics of the infection occurred and lasted for a long time in some places in China in the spring of 2007 and deaths caused by the infection had been reported. The epidemics of EV71 infection occurred again this year in Southern regions of China. Three cases reported in this issue had severe EV71 infection without skin eruption, and had confirmed evidence for pathogenesis. The report of these cases is helpful to pediatricians to recognize the manifestations of this disease.

  • 新生儿红斑狼疮八例分析

    作者:杨群;邵肖梅;曹云;陈超;程国强;施忆赟;周利军

    Objective Neonatal lupus erythematosus (NLE) is an uncommon" passive autoimmune disease, which is associated with transplacental passage of maternal antibodies. It is often misdiagnosed as intrauterine infection or sepsis. The main purpose of this retrospective study was to summarize its clinical manifestations related with pathogenesis.Methods Data of all the NLE neonates, including clinical manifestations, immunochemical evidence of serum antinuclear antibodies (ANA), antibody to Ro/Sjogren's syndrome A ( anti-Ro/SSA), antibody to La/Sjogren' s syndrome B (anti-La/SSB) and anti-dsDNA antibodies in both infants and mothers, and images from head ultrasound and CT scans were analyzed. Follow-up was performed until one and half years of age or when all the clinical abnormalities had been resolved.Results Totally 8 cases (3 males and 5 females ) seen between September 2003 and February 2006 met the diagnostic criteria of NLE, in whom 4 were small for gestational age and one was born prematurely. Mean gestational age was (38.1 ± 1.9 ) weeks, mean birth weight (2605 ± 420) g, mean admission age (22.4 ± 27.7 ) days (2 hours-72 days) and mean age of onset (9.4 ± 12. 1)days (0-28 days). The common clinical manifestations included cutaneous lupus lesions (8 infants ), neural system abnormalities (2 infants ) and congenital heart block (2 infants). Annular, erythematous or desquamative lesions were seen in skin and all disappeared before 6 months of age. One patient presented with third degree atrio-ventricular block and was delivered by cesarean section because of " fetal distress" He did not recover by the end of one and half years follow-up. One infant was hypotonic with delayed neuro-motor development initially and during follow-up with both abnormal neonatal behavioral neurological assessment (NBNA) and imaging findings. Brain CT scan showed generalized low density involving periventricular and deep white matter at one week of age. At the age of one and a half years, he presented with normal mental development index determined by Child Development Center of China (CDCC) infant intelligence mensuration. Other abnormal clinical findings such as hepatosplenomegaly, anemia, thrombocytopenia, cholestasis and elevated liver enzyme activities were all resolved before 6 months of age. Only 3 mothers of the NLE infants were diagnosed as systemic lupus erythematosus (SLE) before parturition and only one received partial therapy. At least anti-Ro/SSA antibody or anti-La/SSB antibody or ANA was found in the affected patients. Seven cases had circulating anti-Ro and/or anti-La antibodies in the mothers and in the newborns, while ANA was positive in seven newborns and in all mothers. All the clinical symptoms disappeared before 18 months ot age except for congenital heart block. No special intervention was applied.Conclusions Serum auto-antibodies should be investigated to rule out NLE when a newborn infant has congenital heart block or rashes or thrombocytopenia, although there is no maternal history of SLE. Central nervous system abnormalities in NLE are likely to be a transient phenomenon and whether it will cause long-term sequelae is uncertain.

  • 北京市儿童与肠道病毒71型感染有关的手足口病伴中枢神经系统症状一例

    作者:李颖;张琪;李杰;任晓旭;朱汝男;钱渊;邓洁

    ASE REPORTA 9-year-and-8-month-old girl experienced intermittent headache of 2 d, fever of 1 d and convulsion of 2 h and was sent to the emergency room (ER). Two days before admission, the girl presented with intermittent headache of obscure cause, which had no fixed site and was tolerable, accompanied by dizziness; she had no change in vision, nor vertigo, nausea, vomiting, cough, nasal discharge or otalgia. One day before hospitalization, the girl became feverish, with the highest body temperature of 38. 8℃ while she had convulsions two hours before the visit to the ER, presenting with binocular gazing, trismus, loss of consciousness, initially noncyanotic face, unaccompanied by extremities rigidity or clonus. Her convulsion could not be controlled after intramuscular injection of 6 mg valium at the local clinic and was transferred to a neighboring secondary hospital 20 min later, where she began to develop extremities clonus, irregular respiration and cyanosis.

  • Citrin缺陷导致的新生儿肝内胆汁淤积症SLC25A13基因三个新突变的识别及诊断

    作者:宋元宗;盛建胜;牛飼美晴;胡務亮;张春花;小林圭子

    Objective Neonatal intrahepatic cholestasis caused by citrin deficiency (NICCD, OMIM 605814 ) is a novel autosomal recessive disease results from mutations in the gene SLC25A13 that encodes for citrin, a liver-type aspartate/glutamate cartier located in the mitochondrial inner membrane. Most of the Chinese NICCD patients diagnosed by genetic analysis had the sameSLC25A13 mutations as Japanese, however, in some cases, the known mutations were not detected. This research aimed to identify novel SLC25A13 mutations in Chinese NICCD patients and to explore the experimental conditions for their genetic diagnosis.Methods Genomic DNA was extracted from blood samples of 3 NICCD patients from Taiwan (P757), Guangdong (P1194) and Hebei (P1443) Province of China, respectively; and all the 18 exons and their flanking sequences of SLC25A13 gene were sequenced. Furthermore, the identified novel mutations were diagnosed by amplification with PCR, digestion with corresponding restriction endonuclease, and agarose gel electrophoresis.Results Three novel mutations identified in SLC25A13 gene of the 3 NICCD patients were an abnormal splicing IVS7-2A>G (P757), a missense A541D (c. 1622C > A, P1194) and a nonsense R319X (c. 955C > T, P1443). The PCR-RFLP procedures for their genetic diagnosis were also established, with specific fragments on electrophoresis after digestion of the PCR products with three different restriction endonucleases Msp Ⅰ, Hpy188Ⅰ and Taq Ⅰ, respectively.Conclusions The three novel mutations in SLC25A13 gene of Chinese NICCD patients were first identified, suggesting that SLC25A13 mutation distributed in Chinese population is somewhat different from that in Japanese. Moreover, the PCR-RFLP diagnostic procedures established in this research provide valuable tools not only for the genetic diagnosis of NICCD but also for further molecular epidemiologic investigations in Chinese population.Acknowledgement We are grateful to all research subjects and their family members for their cooperation, and to many members of medical staff who contributed much to this research. This study was financially supported partially by Guangdong Provincial Research Grant for Science and Technology (No. 2004B50301008) and the Major State Basic Research Development Program of China (No. 2007CB511901 ), and by Grants-in-Aid for Scientific Research (B: Nos. 16390100 & 19390096 ) and for Asia-Africa Scientific Platform Program (AASPP) from the Japan Society for the Promotion of Science.

    关键词: Citrin NICCD SLC25A13 mutation
中华儿科分期目录
期数
2019 01 02
2018 01 02 03 04 05 06 07 08 09 10 11 12
2017 01 02 03 04 05 06 07 08 09 10 11 12
2016 01 02 03 04 05 06 07 08 09 10 11 12
2015 01 02 03 04 05 06 07 08 09 10 11 12
2014 01 02 03 04 05 06 07 08 09 10 11 12
2013 01 02 03 04 05 06 07 08 09 10 11 12
2012 01 02 03 04 05 06 07 08 09 10 11 12
2011 01 02 03 04 05 06 07 08 09 10 11 12
2010 01 02 03 04 05 06 07 08 09 10 11 12
2009 01 02 03 04 05 06 07 08 09 10 11 12
2008 01 02 03 04 05 06 07 08 09 10 11 12 z1
2007 01 02 03 04 05 06 07 08 09 10 11 12
2006 01 02 03 04 05 06 07 08 09 10 11 12
2005 01 02 03 04 05 06 07 08 09 10 11 12
2004 01 02 03 04 05 06 07 08 09 10 11 12
2003 01 02 03 04 05 06 07 08 09 10 11 12
2002 01 02 03 04 05 06 07 08 09 10 11 12
2001 01 02 03 04 05 06 07 08 09 10 11 12
2000 01 02 03 04 05 06 07 08 09 10 11 12
1999 01 02 03 04 05 06 07 08 09 10 11 12
1998 01 02 03 04 05 06 07 08 09 10 11 12
1996 06
1992 04
1991 01 02 03
1989 03

360期刊网

专注医学期刊服务15年

  • 您好:请问您咨询什么等级的期刊?专注医学类期刊发表15年口碑企业,为您提供以下服务:

  • 1.医学核心期刊发表-全流程服务
    2.医学SCI期刊-全流程服务
    3.论文投稿服务-快速报价
    4.期刊推荐直至录用,不成功不收费

  • 客服正在输入...

x
立即咨询