To investigate the molecular mechanism underlying the neuroprotective effect of lithium on cells, in this study, we exposed SH-SY5Y cells to 0.5 mmol/L lithium carbonate (Li2CO2) for 25-50 weeks and then detected the expression levels of some neurobiology related genes and post-translational modifications of stress proteins in SH-SY5Y cells. cDNA arrays showed that pyruvate kinase 2 (PKM2) and calmodulin 3 (CaM 3) expression levels were signiifcantly down-regulated, phosphatase protein PP2A expression was lightly down-regulated, and casein kinase II (CK2), threonine/tyrosine phosphatase 7 (PYST2), and dopamine beta-hydroxylase (DBH) expression levels were signiifcantly up-regulated. Besides, western blot analysis of stress proteins (HSP27, HSP70, GRP78 and GRP94) showed an over-expression of two proteins:a 105 kDa protein which is a hyper-phosphorylated isoform of GRP94, and a 108 kDa protein which is a phosphorylated tetramer of HSP27. These results suggest that the neuroprotective effects of lithium are likely related to gene expressions and post-translational modiifcations of proteins cited above.

Objective To observe the relationship between serum creatine kinase isoenzyme MM sub-bands (CKMM3/MM1 ratio) and the gradation of coronary stenosis and provide a simple, reliable, and economical method for identifying high-risk unstable angina pectoris (UAP). Mehtods Blood samples were drawn at different time after onset of chest pain in 21 patients with UAP and only once in 20 each volunteers for control. CKMM3/MM1 ratio was detected by nonserial buffer agarose gel electrophoresis. CKMB and CK were observed by velocity method. An emergent coronary arteriography was performed as soon as patients were admitted into hospital. Results Patients with UAP were divided into two subgroups:patients with elevated serum enzyme [P( + )] and patients with normal serum enzyme [P( - ) ] according to CKMM3/MM1 ratio ＜ 0.5. Patients with UAP(+)had higher serum CKMM3/MM1 ratios from 0.5 to 12hrs and serum CKMB from 2 to 12 hrs than those with UAP( - ) and control ( P ＜ 0.05) . Serum enzyme concentrations of patients with UAP whose coronary lumen had 90％ or more than 90％ stenosis were significantly higher than those whose coronary lumen had less than 90％ stenosis (P＜0.01) . AnyCKMM3/MM1 ratio was less than 1.0 and CK within the normal range in patients with UAP( + ) group. Conclusions CKMM3/MM1 ratios in patients with UAP can reflect severity of myocardial ischemia. Serum CKMM3/MM1 ratio provides a simple, reliable, and economical method for identifying high-risk UAP.

急性心肌梗死(AMI)早期实验室诊断

急性心肌梗死(AMI)是一种常见且严重危及生命的心脏疾病,在欧美一些国家一直是主要的死因之一.近年来在我国有明显增加的趋势.本病抢救存活的关键是早期诊断、及时抢救.然而诊断急性心肌梗死(AMI)各种不同的生化指标有着不同的应用范围,肌红蛋白(Mb)出现时间早,但窗口期短,CRP具有高灵敏度,但特异度差.肌酸激酶(creat ine kinase,CK)同工酶对AMI早期诊断较AST、LDH特异性高,但此酶出现迟且增高持续时间短,cTnT有更宽的时间窗,cTnI有更高的敏感度和特异度,但出现迟.因此联合CK、CK-MB、Mb、CRP、cTnT、cTnI对急性心肌梗死(AMI)早期实验室诊断价值更高.