您当前的位置：
首页 > 文献资料
所属专业：
B -lymphocytes文献资料
-
应用抑制性消减杂交技术克隆EB病毒致人B淋巴细胞永生化的差异性表达基因
AIM: To clone and identify genes differentially expressed in the EBvirus-transformed human B cell. METHODS: Suppression subtractive hy-bridization was used to construct the library which contains the differentiatelyexpressed cDNAs in EB virus-transformed human B cell. Then the isolatedgenes were cloned and sequenced and identifed by RT-PCR. Nucleic acid ho-mology searches were performed using the BLAST program. RESULTS: By thistechnique, 4 differentiately expressed gene cDNA fragments of EBvirus-transformed human B cell were obtained. CONCLUSION: SSH is aneffective method to isolate differentiately expressed genes.
