首页 > 文献资料
-
基因重叠延伸拼接PCR法钩建骨形态发生蛋白成熟肽真核表达载体的研究
Objective To construct an eukaryote expression vector containing bone morphogenetic protein 2 (BMP 2) mature peptide. Methods Gene splicing by overlapping extension PCR (SOE PCR) method was used to clone BMP2 signal peptide and mature peptide and their fusion fragment.The fusion fragment was cloned into an eukaryote expressing vector pcDNA3.1/myc His(- )A. The sequence of the fusion fragment of BMP2 signal peptide and mature peptide was identified.Results The sequence of the fusion fragment was correct comparing with BMP2 signal peptide and mature peptide published by NCBI.Conclusion The vector pcDNA3.1/myc His(- )A BMP2sm constructed in this experiment was suitable to applying in eukaryotic expression of BMP2.
-
Chemokines play important roles in leukocyte trafficking as well as function regulation. In this study, we described the identification and characterization of a novel CXC chemokine from a human dendritic cell (DC) cDNA library, the ful l-length cDNA of which contains an open reading frame encoding 111 aa with a pu tative signal peptide of 34 aa. This CXC chemokine shares greatest homology with macrophage inflammatory protein (MIP)-2αβ, hence is designated as MIP-2γ.
