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pSVPoMCAT微基因修饰雪旺氏细胞移植治疗实验性脊髓损伤的酶及免疫组化评价
Objective To investigate the effect of microgene pSVPoMcat to modify genetically Schwann cell(SC) on recovery of function after spinal cord injury(SCI).Methods Experimental animals were divided into three groups:the group of microgene pSVPoMcat implanted to genetically(group A),SC implanted group(group B),and the control group(group C),each group having 10 animals.The cortical somatasensory evoked potentials(CSEP) and combined behavioral score(CBS) were continually monitored from operation second week to twelfth week.We used image analysis system to compare the latent period and peak amplitudes of CSEP and count the axon number among the different group.At the same time,electron microscope obsevation was conducted.Results The peak latency and amplitudes of group A,B showed a recovery tendency and it was constant with CBS.The number of regenerated axons of the group A and group B were (388± 163) and (109± 16),respectively(P < 0.01),in line with the observation of electron microscope.Conclusion We conclude that microgene pSVPoMcat to modify genetically SC plays a promoting role in recovery of central function after SCI.
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微基因修饰雪旺氏细胞移植对大鼠脊髓损伤后GAP－43表达的影响
Objective To study the effect of microgene pSVPoMcat implanted to modify schwann cell on growth associated protein－43(GAP－43) expression after spinal cord injury in adult rats.Method Hemisected of the T8 segment of the spinal cord was performed for all the experiment rats.The rats were randomly divided into three groups as follows:Group A with microgene pSVPoMcat implanted to genetically modify SC;Group B with SC implanted ;Group C with hemisection of the spinal cord only.The changes of expression of GAP－43 in spinal cord were observed by immunochemistry with antibodies against GAP－43 .Simultaneous,the combined behavioral scores(CBS)was measured.Result There were not any different(P >0.05)among the three groups in first week and 12 week.There were significant diffeence(P<0.05)among three groups in 2nd,8th,and more dxpression of GAP－43 at the 2nd week in group A.The neurofunctional recovery was best in group A.Conclusion The microgene pSVPoMcat implanted to modify schwann cell can promote the expression of GAP－43 in spinal cord and functional recovery in adults rats after SCI.
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微基因修饰雪旺氏细胞移植对脊髓损伤中枢传导功能的作用
Objective To approach the effect of microgene pSVPoMcat to modify genetically Schwann cells (SC) on central conduction functionafter spinal cord injury (SCI) . Method Experimental animals were divided into three groups: the group of microgene pSVPoMcat implanted to genetically modify SC (group A), SC implanted group (group B), and the control group (group C). The cortical somatasensory evoked potentials (CESP) and combined behavioral score (CBS) were continuous monitored from operation second week to twelfth week Result The result showed that the peak latency and peak amplitudes of group A, B have a recovery tendency and it was constant with CBS. Conclusion We concluded that the microgene pSVPoMcat to modify genetically Sc may play a promotion role in recovery of central conduction function after SCI.
