您当前的位置：
首页 > 文献资料
所属专业：
精子穿透文献资料
-
红芸豆植物凝集素凝胶剂杀精与凝集精子效果的实验研究
目的:探讨红芸豆植物凝集素(RKBL)凝胶剂的杀精和凝集精子的效果.方法:①采用Sander-Cramer方法,即在浓度为5,2.5,1,0.5,0.25,0.1,0.05,0.025,0.01,0.005,0.0025,0.001mg/ml的RKBL溶液中,分别加入等体积精液,观察20s内致死精子的低RKBL浓度及精子凝集的有效浓度;②在“V”型管底部加入等体积RKBL浓度分别为5,2.5,1,0.5,0.25,0.1,0.05,0.025,0.01,0.005,0.0025,0.001mg/ml的凝胶剂,在V型管两侧加入等体积人输卵管培养液(HTF),然后在其中一侧HTF中加入等量精液,观察阻止精子穿过或致死精子的效果.结果:当RKBL的浓度≥1mg/ml时,精子瞬间失活致死,即20s内致死的低剂量为1mg/ml;＞0.01mg/ml的各浓度RKBL可不同程度地凝集精子.在“V”形管中,上述不同浓度的RKBL凝胶剂均能阻止精子穿透;当RKBL浓度≥1mg/ml时,精子在凝胶附近完全失去活动能力.结论:RKBL溶液体外杀精的低浓度为1mg/ml;部分低浓度RBKL能使精子产生不同程度的凝集;各实验浓度的RKBL凝胶剂均能阻止精子穿透.RKBL凝胶剂可有效用于避孕.
