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金属螯合亲和层析对信号分子PLC-γ2 PH结构域的纯化
INTRODUCTION Pleckstrin homology (PH) domains are functional regions in many signal proteins and cytoskeletal associated proteins. Although their function is not very clear, most of the proposed functions involve the localization of proteins on the cell membrane[1] and the mediation of protein-protein interactions[2].