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膜铁转运蛋白Ferroportin 1的研究进展
膜铁转运蛋白Ferroportin 1(2000年发现)在细胞铁的输出中起重要作用.它在成熟的十二指肠绒毛上皮细胞基底面、脾和肝的巨噬细胞、胎盘的合体滋养层细胞等都有表达.经序列分析显示Ferroportin 1具有十个跨膜结构域、一个还原酶位点和一个基底定位信号位点.此外,Ferroportin 1 mRNA转录在5' 非翻译区包含一个铁反应元件.本文对Ferroportin 1的目前研究进行了综述,并阐述了其医学应用前景.
关键词： 铁 Ferroportin 1 铁的输出 -
Previous studies have shown that baicalin prevented iron accumulation after substantia nigra injury, reduced divalent metal transporter 1 expression, and increased ferroportin 1 expression in the substantia nigra of rotenone-induced Parkinson’s disease rats. In the current study, we investigated the relationship between iron accumulation and transferrin expression in C6 cells, to explore the mechanisms of the inhibitory effect of baicalin on iron accumulation observed in Parkinson’s disease rats. Iron content was detected using inductively coupled plasma-atomic emission spectroscopy. Results showed that iron content decreased 41%after blocking divalent metal transporter 1 and ferroportin 1 proteins. After treatment with ferric ammonium citrate of differing concentrations (10, 50, 100, 400 μg/mL) in C6 glioma cells, cell survival rate and ferroportin 1 expression were negatively correlated with ferric ammonium citrate concentration, but divalent metal transporter 1 expression positively correlated with ferric ammonium citrate concentration. Baicalin or deferoxamine reduced divalent metal transporter 1 expression, but increased ferroportin 1 expression in the 100 μg/mL ferric ammonium citrate-loaded C6 cells. These results indicate that baicalin down-regulated iron concentration, which positively regulat-ed divalent metal transporter 1 expression and negatively regulated ferroportin 1 expression, and decreased iron accumulation in the substantia nigra.
