AIM To study the role of lipid peroxides reaction on hepatic ischemia damage and the protective effects ofantioxidants (TMP, co-Q10).MgTHODS Twenty-four adult dogs were performed portacaval shunt and randomly put into 3 groups:group 1 (control group); 8 dogs with portacaval shunt (PCS group); group 2:8 dogs with portacaval shuntand only given antioxidants -TMP (ligustrazine) (PCS + TMP group); group 3:8 dogs with portacaval shuntand given antioxidants in combination with co-Q10, TMP and co-Q10 (PCS + TMP + co-Q10 group). Drugusage: TMP, 50 mg/ kg/ isolation day; coenzyme Q10, 1.5 mg/kg/isolation day; PCS + TMP, given up atthe end of 4 weeks after operation; and PCS + TMP + co-Q10, given up at the end of 8 weeks afteroperation. The experiment indices: super-peroxide dismutase (SOD), malondialdehyde (MDA), ALT,AST, AKP were all measured before operation, and 8 weeks after operation. The liver tissues were obtainedin the 4th week and 8th week respectively after operation and the changes of hepatic structures were observedunder light and electronic microscope.RESULTS There were obvious increase of MDA and decrease of SOD in PCS group, which was remarkablydifferent from pre-operation (P<0.01). In PCS + TMP group, there was no difference between pre-operation and within 4 weeks after operation (P>0.05). But it was remarkably different from PCS group(P<0.01). Beginning from the 5th week after operation, we stopped TMP (ligustrazine), as a result, theobvious increase of MDA, rapid decrease of SOD were found, but not different from PCS group (P >0.05).In PCS+ TMP+ co-QlO group, MDA increased slightlly, and SOD decreated, but it was superior to that inPCS and PCS + TMP groups. The data in PCS + TMP + co-Q10 group had statistical significance compared tothat in PCS+ TMP group (P<0.05) during 4 weeks after operation; but from the 5th to 8th week, there wasa remarkable difference (P<0.01). The results of ALT, AST and AKP showed remarkable differencebetween PCS + TMP + co-Q10, PCS group (P<0.01) and PCS + TMP compared to PCS + TMP + co-Q10(P<0.05). After stopping TMP, PCS + TMP were compared with PCS + TMP + co-Q10 (P<0.01). Thechanges in liver structures were fatty degeneration, atrophy and necrosis; decrease in rough surfacedendoplasmic reticulum (PER), mitochondrial swelling, partial mitochondria fusion, disappearance ofmitochondrial crista, diminution of Golgi body. The structures of liver cells in PCS + TMP and PCS + TMP +co-Q10 groups were superior to that in PCS group; but in the 8th week after operation, the liver structuralchanges bad no difference between PCS + TMP and PCS group. The results of light electronic microscopy inPCS + TMP + co-Q10 were better than that in PCS and PCS + TMP groups.CONCLUSION The over-activated lipid peroxides reaction may be one of the important factors of hepaticischemia damage after portacaval shunt; combined use of the antioxidants can enhance the protection fromthe hepatic ischemia damage.

先天性门-腔静脉分流畸形的影像分析

先天性门-腔静脉分流(congenital portacaval shunt,CPCS)是由于门静脉系统发育异常所致的一种十分罕见的肝外门静脉系统血液分流人体静脉系统的畸形,Abernethy于1793年首次报道[1].目前国外文献共报道50例,其中Ⅰ型42例,Ⅱ型8例.国内文献尚未见报道.我院发现1例Ⅱ型CPCS畸形并肝内局灶性结节增生(focal nodular hyperplasia,FNH)的病例,结合文献对其进行分析.