AIM To obserwe tb e effect of pentagastrin (G-5) on IL-1β induced inhibition of insulin secretion in newbornrat islet of Langerhans.METHODS Islets of Langerhans of 3- to 5-day-old rats were isolated by collagenase digestion. The isletswere maintained free-floating in culture medium RPMI-1640, containing 10% (V/V) calf serum, anddistributed randomly in 96-well plastic plates (6 wells in each group). There are 15 islets per well in 0.2 mLculture medium. The islets were kept at 37℃ in mixed gases of 5% CO2 and 95% humidified air for the timerequired by the experimental design. Three experiments were performed in this study. (①) IL-13 inducedinhibition of insulin secretion in isolated islets of Langerhans. (②) Effect of G-5 on IL-lβ induced inhibition ofinsulin secretion. ③ Effect of G-5 on the functional repair of islet B-cells inhibited by IL-1β. Accumulatedand glucose stimulated insulin secretion was measured by radioimmunoassay in all studies. Data are presentedas ~ ± s. Differences between groups were analyzed using the Student's t test. P <0.05 was consideredsignificant.RESULTS The function of islet B-cells, which has been received 1L-1β treated for 24 hours, was dose-dependently inhibited. The accumulated and glucose stimulated insulin secretion was significantly lower thanthat of the control group (P<0. 05). The inhibitory effect of IL-1β on islet B-cells can be partially reversedby G-5. Accumulated and stimulated insulin secretion of G-5 0.6 ng/mL and 0.8 ng/mL groups wassignificantly higher than that of IL-1β treated alone group (P<0.05). The function of islet B-cells, whichreceived IL-iβ treatment for 24 hours, could partially recover after G-5 treatment for another 24 hours. Butaccumulated and glucose-stimulated insulin secretion in groups with G-5 treatment for 10 hours groups had nosignificant difference as compared with IL-1β treated alone group (P>0.05).CONCLUSION The present results indicate that G-5 may have a protective effect against the toxicity of IL-1β on islet B-cells.

儿童肩胛骨Langerhans组织细胞增生症1例

发生于肩胛骨的Langerhans组织细胞增生症(Langerhans cell histocytosis,LCH),未见文献报道,本文报道1例如下.男, 5岁.因拍片发现左肩胛骨病变1个月收住.患者患枕骨肿块1年,外院枕骨CT、MRI示枕骨局灶性溶骨性病变,并周围软组织增生反应,术前常规拍胸片发现左侧肩胛骨溶骨性病变,行枕骨病变切除术,术后病理检查报告为表皮样囊肿,恢复良好.为继续治疗肩胛骨病变转入本院.