A case of a fibrous histiocytoma (FH) of the larynx in a 54-year-old male is reported. Laryngeal fibrous histiocytoma is uncommon. The case recurred several times over 4-year period. Its pathology is described including arguments on potential malignancy and the way of management.

全喉切除后发音功能重建术式探讨

Objective To establish a surgery model that can prevent stenosis of trachea stoma and reconstruct vocal function by stoma with tracheal-esophagus flaps.Methods Vocal reconstruction operations of 29 laryngectomized patients from 1994 were performed with the first stage mucosal valvular shunt of tracheo-esophagus and Griffith tracheostoma enlargement method.Results In these 29 cases,26 cases achieved voice reconstruction.In them,there were 2 cases needed cannulation for 3 months.The others didn't need cannulation and no stricture was found after followed them up for more than one year.Conclusion The vocal reconstruction operations with Griffith tracheostoma enlargement method and first stage mucosal valvular shunt of tracheo-esophagus is a good method to treat laryngeal carcinoma and resume laryngeal function.

P27基因对人喉癌细胞抑制作用的研究

Objective To explore the effect of p27 gene on the growth inhibition of laryngeal carcinoma cell line Hep 2. Methods The p27 cDNA was transfected into human laryngeal carcinoma cell line Hep 2 cells with lipofectamine. The cell cycles were observed by means of FCM assay. p27 expression was detected by dot blot hybridization and Western blot. Results Expression of p27 in Hep 2 was identified by Dot blot and Western blot analyses.the growth rate of Hep 2 transfected with p27 gene was markedly suppressed. Cell cycle analysis by flow cytometry show that the number of cells in G0～ G1 phase of Hep 2 cells was significantly increased while cells in S and G2+ M phase was decreased compared with that of the control Hep 2 cells. Conclusion Transduction of p27 gene into lower expression cancer cells can restore its suppressive effect on cell growth by arrest of cell cycle at G1 phase.