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抑制脑血栓形成的硫代寡核苷酸的细胞摄取率和亚细胞分布
AIM:To explore the membrane permeability, enzyme tolerance, and stability of phosphorothioate oligodeoxynucleotide(P ODN) which can inhibit cerebral thrombosis.METHODS:(1)Oligodeoxynucleotide was synthesized with phosphoramidite method in solid phase,3' end was modified with thiophosphoter.(2)Uptake and subcellular distribution of P ODN.P ODN was given directly.We observed total uptake rate of P ODN by ECV304 cells and subcellular distribution of P ODN.RESULTS:(1)ECV cells uptake more P ODN with increase of concentration,the uptake rate was about 10.8% - 11.9% .(2)Subcellular distribution of P ODN:P ODN was mainly distributed in nucleus especially nucleus sediments(69.3% - 77.2% )(P< 0.05).CONCLUSION:P ODN suppressing cerebral thrombosis has favorable transmembrane ability,enzyme resistance and stability.
