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Objective: To investigate the effect of anthopleurin-Q (AP-Q) on the intracellular Ca2+ concentration ([Ca2+ ]i) in cultured cortical astrocytes of rats. Methods: The [Ca2+ ]i was monitored by calcium imaging with Ca2+ sensitive fluorescent probe fura-2. Results: A concentration of 300 nmol/L AP-Q increased the [Ca2+ ]i in astrocytes by (136.98%±35.63%) (n=28),when compared with the baseline level. Furthermore, the elevation of [Ca2+]i was prevented by extracellular calcium free solution or when the extracellular Na+ was replaced by NMDG+ , and was decreased by Ni+ ,a non-specific antagonist of Na+/Ca2+ exchanger. Conclusion: AP-Q induced the intracellular [Ca2+ ]i elevation in cultured rat cortical astrocytes via activating the reverse mode of Na+/Ca2+ exchanger. AP-Q may be a useful tool to develop experimental model of seizures.
