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HPLC测定除痰止嗽丸中的桔梗皂苷D、桔梗皂苷E、白花前胡甲素和白花前胡乙素
目的:采用高效液相色谱法测定除痰止嗽丸(CTZSW)中桔梗皂苷D、桔梗皂苷E、白花前胡甲素和白花前胡乙素的含量.方法:Hypersil C18色谱柱(4.6 mm×150 mm,5 μm),流速1.0 mL·min-1,流动相乙腈-水(35∶65)(桔梗皂苷D和桔梗皂苷E),ELSD漂移管温度105℃,载气(N2)流速2.6 SLPM· min-;流动相乙腈-甲醇-水(40∶ 30∶ 30)(白花前胡甲素和白花前胡乙素),检测波长321 nm.结果:桔梗皂苷D和桔梗皂苷E分别在0.041 3~0.826 0μg(r =0.999 7),0.079 7~1.5940μg(r =0.999 8)进样量与峰面积呈良好的线性关系,平均加样回收率分别为98.32%,98.94%,RSD(n =6)分别为0.80%,0.93%;白花前胡甲素和白花前胡乙素分别在0.154 9 ~3.098 0μg(r =0.999 4),0.051 7 ~1.034 0 μg(r =0.999 8)进样量与峰面积呈良好的线性关系,平均加样回收率分别为98.72%,99.15%,RSD(n =6)分别为0.86%,0.95%.结论:该方法测定结果准确、灵敏、重复性好;可用于除痰止嗽丸的质量评价.