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Objective To perform pathological observation and etiological identification of specimens collected from dairy cows, beef cattle and dogs which were suspected of rabies in Inner Mongolia in 2011, and analyze their etiological characteristics.
Methods Pathological observation was conducted on the brain specimens of three infected animals with Hematoxylin-Eosin staining, followed by confirmation using immunofluorescence and nested RT-PCR methods. Finally, phylogenetic analysis was conducted using the virus N gene sequence amplified from three specimens.
Results Eosinophilic and cytoplasmic inclusion bodies were seen in neuronal cells of the CNS; and rabies non-characteristic histopathological changes were also detected in the CNS. The three brain specimens were detected positive. N gene nucleotide sequence of these three isolates showed distinct sequence identity, therefore they fell into different groups in the phylogenetic analysis. N gene in the cow and dog had higher homology with that in Hebei isolate, but that in the beef cattle had higher homology with that in Mongolian lupine isolate and Russian red fox isolate.
Conclusion Rabies were observed in the dairy cow, beef cattle and canine in the farm in Inner Mongolia, in 2011, which led to a different etiologic characteristics of the epidemic situation.关键词： Rabies Cow Histopathology Phylogeny -
低敏配方在牛奶蛋白过敏患儿中的应用
近年来,食物过敏(food allergy,FA)的发病率呈持续上升趋势.据报道,每12名婴幼儿中就有1名患有食物过敏,其中牛奶蛋白过敏(cow's milk protein allergy,CMPA)为多见.CMPA是指由牛奶蛋白引起的异常或过强的免疫反应.现有资料显示CMPA的发病率约为2%～3%,年龄多集中在2岁以下~([1]).
