Mutations of integrin-interacting protein Kindlin-1 cause Kindler Syndrome and deregulation of Kindlin-1 is implicated in human cancers. The Kindlin-1-related diseases are confined in limited tissue types. However , Kindlin-1 tissue distribution and the dogma that governs Kindlin-1 expression in normal human body are elusive. This study examined Kindlin-1 expression in normal human adult organs, human and mouse embryonic or-gans by immunohistochemical analyses. We identified a general principle that the level of Kindlin-1 expression in tissues is tightly correlated with the corresponding germ layers from which these tissues are originated. We com-pared the expression of Kindlin-1 with Kindlin-2 and found that Kindlin-1 is highly expressed in epithelial tissues derived from ectoderm and endoderm, whereas Kindlin-2 is mainly expressed in mesoderm-derived tissues. Like-wise, Kindlin-1 was also found highly expressed in endoderm/ectoderm-derived tissues in human and mouse em-bryos. Our findings indicate that Kindlin-1 may play an importance role in the development of endoderm/ectoderm related tissues.

荧光蛋白、超顺磁氧化铁双标胎鼠神经干细胞建立

目的 建市绿色荧光蛋白(EGFP)、超顺磁氧化铁(SPIO)双标大鼠胚胎中脑神经干细胞.方法 以质粒pEGFPN1转染大鼠胚胎中脑神经干细胞后用SPIO标记.荧光显微镜观察EGFP表达情况;普鲁士蓝染色、透射电镜鉴定SPIO标记;体外诱导分化,免疫细胞化学鉴定其分化能力.结果 基因转染12 h后EGFP开始表达;普鲁十蓝染色显示SPIO标记满意,透射电镜显示SPIO位于吞饮小泡和胞质内;体外诱导分化研究表明EGFP、SPIO双标不影响其增殖与分化.结论 成功建立了EGFP、SPIO双标大鼠胚胎中脑神经干细胞.

大鼠内耳发育过程表皮生长因子的表达（英）

Since the two independent discoveries in 1987 indi-cated that hair cells within cochlea of bircls were capableof spontaneous regeneration, recent observations havedemonstrated that, in response to injury, mammals pos-sess a limited capacity of regenerating inner ear sensoryepithelia, but the mechanism of regeneration is still tm-known. Lewfebvre (1993) and Lambert (1994) reportedrespectively that retininoic acid (RA) and transforminggrowth factor alpha (TGF-α) could stimulate hair cell re-generation in the mammalian inner ear. These studies in-dicated that the cellular factors participating the normaldevelopment of cells could play a critical role in the re-generative process of hair cells.Epidermal growth factor (EGF) plays a critical rolein proliferation and differentiation of normal cells and tis-sues and it is extensively expressed in many mature andembryonic tissues.