Enhancer of zeste homolog 2 ( EZH2 ) is a key epigenetic regulator that catalyzes the trimethyla-tion of H3K27 and is modulated by post-translational modifications (PTMs). However, the precise regulation of EZH2 PTMs remains elusive. We, herein, report that EZH2 is acetylated by acetyltransferase P300/CBP-associat-ed factor (PCAF) and is deacetylated by deacetylase SIRT1. We identified that PCAF interacts with and acetylates EZH2 mainly at lysine 348 (K348). Mechanistically, K348 acetylation decreases EZH2 phosphorylation at T345 and T487 and increases EZH2 stability without disrupting the formation of polycomb repressive complex 2 ( PRC2 ) . Functionally, EZH2 K348 acetylation enhances its capacity in suppression of the target genes and promotes lung cancer cell migration and invasion. Further, elevated EZH2 K348 acetylation in lung adenocarcinoma patients pre-dicts a poor prognosis. Our findings define a new mechanism underlying EZH2 modulation by linking EZH2 acety-lation to its phosphorylation that stabilizes EZH2 and promotes lung adenocarcinoma progression.

Objective: To investigate the relationship between the prognosis of Epithelial Ovarian Cancer (EOC) and its ascites. Methods: Retrospectively analysis is performed for the clinical, pathological and followed up data of 101 in-patients suffering from epithelial ovarian cancer and operated with tumor debulking surgery in our hospital from January 1986 to December 1993. The patients was divided into two groups based upon the first laparotomy finding with ascites(62) or without(39). Age average, cell type, advanced proportion and survival rate of the patients are evaluated by a c2 test. Results: For age average and cell type, no statistical difference was noted. However, there were more advanced cases in ascites group than in the other (P<0.01). The 3-, 4- and 5-year survival in the no-ascites group were 87.02%, 73.42%, 57.10% respectively compared with 65.02%, 38.66%, 28.12% in the ascites group. The 5-year survival rate of stage I, II,III, IV patients in no-ascites group are 77%, 70%, 41.1%, 0 respectively, compared with that of 60%, 56.8%, 15.46%, 0 respectively in the ascites group. The results shows that 3-, 4-, and 5-year survival in no-ascites group were significantly higher than those in ascites group(P<0.01). Conclusion: Presence of ascites is a factor of poor prognosis for EOC.

Objective. To report the magnetic resonance imaging (MRI) findings of acute cervical central cord syndrome and to determine their correlation with the prognosis. Methods. MRI findings of 35 patients with acute central cord syndrome were studied and compared with the recovery rate of ASIA score at presentation and in follow-up. Results. MRI data demonstrated spinal cord compression for 32 patients, spinal cord swelling for 16 patients, and abnormal signal intensity within the spinal cord for 19 patients, including 14 with edema and 3 with hematoma. No significant difference of the recovery rate was noted between the patients treated nonoperatively and operatively (P >0.05). There was a significant inverse correlation between the recovery rate and the degree of spinal cord compression as shown in MRI scans (P<0.01). The presence of hematoma in MRI scans was associated with poor prognosis, as demonstrated by a significant difference of the recovery rate (P< 0.01) among the patients with normal intensity, edema and hematoma within the spinal cord. Conclusions. MRI scans provide an efficient assistance for decision-making and accurate prognostic information regarding neurological function, and therefore should routinely be performed within the early phase of acute central cord syndrome.

In this special issue on hematology, four original research papers in the field of hematology from different institutions over China are presented. Zhao Chunhua et al from the National Key Laboratory of Hematology in Tianjing report on a primary CML cell model. By transfecting the bcr-abl gene into human CD34+ cells, a primary human hematopoietic stem/progenitor cell model was well developed with P210bcr/abl expression, elevation and relocation of P27kip, MDR-1 Pgp increment and other typical CML characteristics. This primary CML stem/progenitor cell model will be useful in studying the role of intracellular signal mechnisms in the pathogenesis of CML.

蛛网膜下腔出血并发脑血管痉挛预防的实验与临床研究进展

Cerebral vasospasm(CVS),a common complication of subarachnoid hemorrhage(SAH),is caused by many factors and associated with poor prognosis of SAH.CVS generally occurs 3-4 days after the initial SAH and peaks at 5-7 days.

Objective:To investigate the the correlation between lymphatic vascular invasion (LVI) and prognosis in T3/T4 gastric cancer after D2 resection, and establish an optimal classification of staging system. Methods: From Jan 2000 to Sep 2010, a total of 1, 283 T3/T4 gastric cancer patients undergoing D2 resection were enrolled. Univariate and multivariate analysis were used to investigate the prognostic value of gastric cancer patients. Homogeneity, discriminatory ability, and monotonicity of gradients of hypothetical N stage and UICC N stage were compared using linear trendχ2, likelihood ratioχ2 statistics, and Akaike information criterion (AIC) calculations.Results:Multivariate analysis identified LVI was an independent prognostic factor. The 3.5-year overall survival were worse in patients with LVI than those without LVI (P<0.001). LVI was corporated into N3b stage performed the optimum prognostic stratification, together with better homogeneity, discriminatory ability and monotonicity of gradients. Conclusion:LVI is an independent prognostic factor for T3/T4 gastric cancer atfer D2 resection, and may be considered to be incorporated into the UICC N3b stage.

Objective:Lymphadenectomy has been increasingly regarded as standard surgical procedure for advanced gastric cancer (GC), while necessity No.14v lymph node dissection for lower GC is still controversial.Methods: A total of 311 GC patients receiving D1+ (D1+7, 8a, 9) or D2 plus No.14v lymph node dissection in our center were enrolled. Patients were categorized into two groups based on No.14v lymph node status: positive group (PG) and negative group (NG).Results:Fifty patients (16.1%) had No.14v lymph node metastasis. Metastasis to No.4d, No.6 lymph node and distant metastasis were independent variables affecting No.14v lymph node metastasis. Patients with positive No.14v lymph node had a significant lower overall survival (OS) rate than those without (3-year OS, 34.0% vs. 67.0%,P<0.001).Conclusion:GC patients with positive No.4d and No.6 lymph node often metastasis to No.14v lymph node. Status of No.14v lymph node was an independent prognostic factor for GC staged TNM III. Patients with positive No.14v lymph node usually have a poor prognosis, while such patients without distant metastasis may beneift from a curative surgery.

Objective:MiR-34 is a prospective prognostic marker in cancer, but its association with GC is still controversial. To assess the clinicopathological and predictive significance of miR-34 expression in GC we performed this meta-analysis.Methods: Data were extracted from eligible studies.Meta-analysis was performed with STATA12.0sotfware. Results:A total of 7 papers (806 cases) were included. A meta-analysis of clinicopathological variables revealed a correlation between miR-34 expression and differentiation grade (OR =0.95, 95% CI, 0.74~1.22,P<0.05). However, no significant associations were found in other variables. Subgroup analysis by stratified detection methods showed no significative associations between the expression of miR-34 and clinicopathological variables in GC. The correlation between miR-34 expression and poor prognosis was statistically signiifcant (HR =1.52, 95% CI, 1.14~1.90,P<0.05). Conclusion:This study shows that miR-34 is a potential prognostic marker of GC.Future research with larger amount of samples should be developed to investigate thoroughly the role of miR-34 expression in GC.

Tip30基因甲基化与肝细胞癌临床预后的关系

肿瘤细胞DNA甲基化紊乱常表现为癌基因去甲基化和抑癌基因高甲基化,即抑癌基因功能失活是通过表观遗传调控抑制,而不是通过基因缺失或突变.既往研究在肝细胞癌中p16、SOCS-1、TFPI-2、E-cadherin、RASSFIA和NOREIB等抑癌基因常常表现为高甲基化状态,这些基因的高甲基化状态与肝细胞癌的发生、发展关系密切.