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Current research on bone marrow stem cell transplantation and autologous or xenogenic nerve transplantation for peripheral nerve regeneration has mainly focused on the repair of peripher-al nerve defects in rodents. In this study, we established a standardized experimental model of radial nerve defects in primates and evaluated the effect of repair on peripheral nerve injury. We repaired 2.5-cm lesions in the radial nerve of rhesus monkeys by transplantation of autografts, acellular allografts, or acellular allografts seeded with autologous bone marrow stem cells. Five months after surgery, regenerated nerve tissue was assessed for function, electrophysiology, and histomorphometry. Postoperative functional recovery was evaluated by the wrist-extension test. Compared with the simple autografts, the acellular allografts and allografts seeded with bone marrow stem cells facilitated remarkable recovery of the wrist-extension functions in the rhesus monkeys. This functional improvement was coupled with radial nerve distal axon growth, a higher percentage of neuron survival, increased nerve fiber density and diameter, increased myelin sheath thickness, and increased nerve conduction velocities and peak amplitudes of compound motor action potentials. Furthermore, the quality of nerve regeneration in the bone marrow stem cells-laden allografts group was comparable to that achieved with autografts. The wrist-extension test is a simple behavioral method for objective quantification of peripheral nerve regeneration.
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骨髓干细胞移植治疗缺血性心脏病的研究与进展
缺血性心脏病(ischemic heart disease)发展到晚期大多导致心力衰竭,严重影响着心脏病患者的预后及生活质量。近年来,虽然药物治疗、介入技术和外科手术水平不断提高,使急性心梗的早期病死率下降,然而循证医学表明,在成功接受血运重建治疗的心肌梗死患者中,仍有超过30%的患者经历着心梗后室壁变薄、室腔扩大的心室重构过程。因为早期血管重建只能挽救冬眠缺血的心肌,而己经坏死心肌必将被纤维结缔组织替代,形成疤痕,终发展为慢性缺血性心力衰竭。骨髓干细胞因具有在体外有良好的增殖能力,可以在心肌内微环境下分化为损伤组织所需要的心肌细胞,以及其自分泌、旁分泌等作用且无移植后免疫排斥反应等优点,利用骨髓干细胞移植治疗缺血性心脏病的研究越来越多。现就骨髓干细胞的分类、途径、作用机制、存在的问题及前景等作简要介绍。
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网织血小板比率测定在异基因骨髓干细胞移植中的应用
网织血小板比率(immature platelet fraction,IPF)的测定,以往均采用流式细胞仪法,但其所用仪器和试剂均较昂贵,且耗时较长,也需要专业人员,因此未能作为常规项目开展.目前,软件升级后的XE-2100血液分析仪可进行IPF的检测,我们采用这种方法对我院异基因骨髓干细胞移植(allogenic bone marrow stem cells,Allo-BMS)后患者IPF的情况进行了监测,现报告如下.
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