Protective Effect and Mechanism of TGF - β1 on Intestinal Mucosa against IR Injury in Rats

Objective To investigate the protective effect and mechanism of TGF - 1 on intestinal mucosa against ischemia-reperfusion (I/R) injury, so as to provide new methods of clinical prevention from and dealing with intestine I/R injury and gut - derived multiple organ dysfunction syndrome(MODS). Methods 30 SD rats were randomly divided into 3 groups ( n = 10 each), A: namely sham - operation group ( group A) : superior mesenteric artery ( SMA ) was only isolated but not blocked; B: control group (group B), ischemia for 60 minutes, then reperfusion for 120 minutes, and administered with normal saline solution (NS) 0. 5ml through SMA 10 minutes before SMA blockage; C: experimental group (group C) : animals underwent the same procedure with group B except for taking TGF-β1 instead of NS. The contents of intestinal mucosa Chiu's sore, bacteria translocation, intestinal mucosa cellular apoptosis index, expression of bax mRNA and bcl - 2 mRNA and protein were measured. Results Compared with group A, the results from group B are as follows, the Chiu's score significantly increased, bacteria translocation increased, intestinal mucosa cellular apeptosis rate significantly increased, level of bax mRNA and Bax protein significantly increased, while level of intestinal mucosal bcl -2 mRNA and Bcl -2 protein significantly decreased. All the changes in group B were reversed significantly in group C. Conclusion TGF - 1 exerts a protective effect on the gut barrier against intestine I/R injury, perhaps via reversing injury - induced downregulation in bcl2 transcription and upregulation in bax transcription, with the result in modulating bcl -2 and bax protein expression and downregulation of the susceptibility to apoptosis of intestinal epithelial cells.