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Objective. This study characterized the activation of platelet integrin aⅡbβ3 induced by two anti-human platelet te-traspanin monoclonal antibodies(mAbs),HI117 and SJ9A4. Methods. Using 125I-labeled human fibdnogen(Fg), specific Fg binding to human platelets induced by HI117 and SJ9A4 was measured as indication of activation of platelet integrin αⅡbβ3 by the two mAbs. Results. H1117 and SJ9A4( 10μg/ml and 20μg/ml) induced evident specific Fg binding to human platelets, sug-gesting that the two mAbs evoked activation of platelet integrin αⅡbβ3. Further study indicated that HI117 and SJ9A4 induced integrin t Ⅱ 1β3 activation independent of platelet Fc-receptors, and that HI117 and SJ9A4-induced integrin αⅡbβ3 activation was inhibited by sphingosing, aspirin, apyrase, and/or PGI2. Conclusion. The anti-platelet tetraspanin(CD9)mAbe,HI117 and SJ9A4, can induce platelet integrin αⅡbβ3 act-vation independent of Fc-receptors. Three signaling pathways,i.e.thromboxane,secreted ADP, and cAMP pathways may be involved in the process, with protein kimse C activation presumably being the comtmon step of the three pathways.
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血吸虫疫苗研究新进展
血吸虫病是一种防治难度极大的人畜共患寄生虫病,在全球范围内其患病人数仅次于疟疾,是第二大热带寄生虫病.数十年来,血吸虫疫苗研究逐步从死疫苗、减毒活疫苗发展到基因工程亚单位疫苗.随着血吸虫分子生物学领域的飞速发展,血吸虫基因组学、转录组学和大部分蛋白组学研究基本完成,人们发现了一批新的疫苗候选分子.本文综述了血吸虫疫苗新近的研究进展,着重描述了tetraspanin为代表的膜蛋白疫苗.
关键词： 血吸虫病 疫苗 膜蛋白 tetraspanin
