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Objective To investigate the distribution and clonality of T cell receptor (TCR) Vβ repertoire in patients with acute monoblastic leukemia (AML-M5).Methods Expression of the TCR Vβ repertoire was analyzed using reverse transcription-polymerase chain reaction (RT-PCR), which amplified the complementarity determining region 3 of 24 TCR Vβ genes in peripheral blood from 9 cases with acute myclogenous leukemia subtype 5 or acute monoblastic leukemia (AML-M5). PCR products were further studied by genescan analysis to identify T cell clonality. Results Expression of 1-10 Vβ subfamilies was found in samples from 9 patients. Genescan analysis showed that some Vβ subfamily products from 8 of 9 cases contained an oligoclonal peak. Oligoclonal T cells of the Vβ2 subfamily could be found in 6 patients with AML-M5. Conclusions T cell clonality expansion was found in AML-M5 cases and were tendentious in the Vβ2 subfamily, suggesting a the specific immune response for leukemia cell (M5) associated antigen and may display antileukemia activity.
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CⅡ诱导RA患者关节滑液中自身反应性T细胞的免疫学特性分析
目的:探讨CⅡ诱导的RA患者关节滑液中自身反应性T细胞免疫学特性.方法:分离RA患者关节滑液中SFMC,用CⅡ刺激后分析反应性的T细胞频率;用ELISA方法检测CⅡ刺激SFMC后上清中的IFN-γ和IL-4的含量及通过流式细胞检测SFMC表面标志的表达;应用半定量PCR分析外周血及滑膜液中TCR Vβ基因表达格局.结果:SFMC中CⅡ反应性的T细胞频率高于PBL;IFN-γ的含量在CⅡ刺激SFMC后上清中较高,而在未刺激中较低 (P＜0.01),在PBL中更低 (P＜0.05); SFMC上CD8及CXCR3表达多于PBMC(P＜0.05);外周血T细胞表达绝大多数Vβ基因,而滑膜液中TCR Vβ基因取用呈现明显的不均衡性.结论:CⅡ反应性T细胞在SFMC中占优势,可能是介导RA发病的主要原因.
关键词： 类风关 自身反应性T细胞 TCR Vβ gene CⅡ
