AbstrAct Objective:Our group has previously observed that in patients with small-cell lung cancers (SCLCs), the expression of a tumor antigen, glioma big potassium (gBK) ion channel, is higher at the time of death than when the cancer is ifrst treated by surgical resection. This study aimed to determine whether this dichotomy was common in other potential lung tumor antigens by examining the same patient samples using our more extensive proifle analysis of tumor-antigen precursor protein (TAPP). We then tested the hypothesis that therapeutic intervention may inadvertently cause this increased gBK production. Methods:SCLC samples (eight surgical resections and three autopsy samples) and three control lungs were examined by quantitative real-time polymerase chain reaction for 42 potential TAPPs that represent potential T-cell-mediated immunological targets. Results:Twenty-two TAPP mRNAs displayed the same profile as gBK, i.e., more mRNAs were expressed at autopsy than in their surgical counterparts. B-cyclin and mouse double minute 2, human homolog of P53-binding protein were elevated in both autopsy and surgical specimens above the normal-lung controls. When HTB119 cells were incubated with doxorubicin, gBK was strongly induced, as conifrmed by intracellular lfow cytometry with a gBK-speciifc antibody. Conclusion:Our findings suggested that more immunological targets became available as the tumor responded to chemotherapy and proceeded toward its terminal stages.

不同浓度氯胺酮对成人辅助性T淋巴细胞分化的影响

Objective To investigate the effects of different concentrations of ketamine on the differentiation of human T helper (Th) cells.Methods Twenty ASA Ⅰ patients (aged 20-60 years) undergoing elective operation under general anesthesia were enrolled in this study.Peripheral venous blood samples were taken before anesthesia.Peripheral blood mononuclear cells were isolated and divided randomly into three groups (n =20 each):being incubated in the presence of 0.9％ NaCl (group C),2.5 μg/ml ketamine (group K1) and 25.0 μg/ml ketamine (group K2),respectively,for 24 hours,and were then stimulated with phytohaemagglutinin for 48 hours,respectively.The percentages of Th1 and Th2 cells were detected by four-color fluorescence flow cytometry.The Th1/Th2 ratio was calculated.The levels of interleukin-2 (IL-2),IL-4,IL-6,IL-10,immunoreactive fibronectin-γ (IFN-γ) and tumor necrosis factor-alpha (TNF-α) in the supernatant were determined by cytometric bead array.Results There was no significant difference in the levels of IL-2,IL-4,IL-6,IL-10,IFN-γ and TNF-α in the supernatant,the percentages of Th1 and Th2 cells and the ratio of Th1/Th2 among groups C,K1 and K2(P＞ 0.05).Conclusion The sedative and anesthetic concentrations of ketamine exert no effect on the differentiation of human Th cells in vitro.

人类免疫缺陷病毒感染者不同疾病阶段中自然杀伤细胞和γδT细胞的改变

目的 了解NK细胞和.γδT细胞在HIV/AIDS患者不同疾病阶段中的改变特点,探讨其在AIDS发病机制中的作用.方法 以311例未接受过抗HIV治疗的HIV/AIDS患者为研究对象,经流式细胞仪检测患者外周血CD4+T淋巴细胞、NK细胞和γδT细胞比例及计数,根据CD4+T淋巴细胞<0.20×109L、(0.20～0.35)×109L及>0.35×109/L.将病例分为低、中、高CD4+T淋巴细胞组,进行组间比较.Mann-WhitneyU检验和Kruskal-Wallis检验进行两组和多组独立样本秩和检验,Spearman和Pearson检验进行相关性分析.结果 HIV/AIDS患者外周血NK细胞比例及计数的中位数分别为8.4%和103×106L,γδT细胞比例及计数中位数分别为3.4%和41×106L,均明显低于健康对照组(Z=-5.029,Z=-7.723,Z=-2.437,Z=-6.063;均P<0.01).低、中、高CD4+T淋巴细胞组CD4+T淋巴细胞计数中位数分别为0.062×109L、0.276×109L、0.482×109L,NK细胞计数分别为89×106L、97×106L、146×106L,低、中CD4+T淋巴细胞组间的NK细胞计数差异无统计学意义,但均显著低于高CD4+T淋巴细胞组(Z=-3.392,P=0.001;Z=-4.849,P<0.01).低、中、高CD4+T淋巴细胞组γδT细胞计数中位数分别为29×106L、43×106L、59×106L,两两之间比较均差异有统计学意义(P<0.05).结论 HIV感染后外周血NK细胞和γδT细胞数量降低程度存在差异.

溃疡性结肠炎患者肿瘤坏死因子α与T细胞亚群的变化及其相关性研究

目的和方法:探讨溃疡性结肠炎(UC)之肿瘤坏死因子α与T细胞亚群的变化及其相关性,测定25例溃疡性结肠炎患者外周血单个核细胞(PBMC)经培养于体外自发和经LPS诱生的TNFα含量及其外周血T细胞亚群百分率和比值.结果:①UC组与对照组自发产生的TNFα含量间无明显差异(P＞0.05).LPS诱生后TNFα含量UC组明显低于对照组(P＜0.05).②UC组与对照组CD3细胞百分率无明显差异(68.86%±4.10%vs70.10%±5.04%,P＞0.05).UC组CD4细胞显著低于对照组(32.72%±6.06%vs41.15%±7.26%,P＜0.01),而其CD8细胞则高于对照组(24.96%±4.02%vs21.88%±4.17%,P＜0.05),使得其CD4/CD8的比值明显低于对照组(P＜0.01).③两组诱生后的TNFα含量与其CD4、CD8、CD4/CD8之间均无相关性.结论:UC患者TNFα的诱生能力低下,T细胞亚群数量及比值异常,TNFα诱生水平与T细胞亚群的变化间无相关性.