Objective:The aim of our study was to investigate the ef ects of the anti-tumor composition of the acetoacetate extract of Vitex Negundo Seed (EVn-50) on the growth of human cervical carcinoma HeLa cells xenografts in nude mice and its possible molecular mechanism. Methods:Models of human cervical cancer HeLa cells xenografts transplanted subcuta-neously in nude mice were established and randomly divided into 7 groups (each group including 5 nude mice):saline group, Taxol group, EVn-50 group, comp-6 group, comp-7 group, comp-8 group and comp-10 group. The volume and weight of Xe-nograts were observed and compared. The alteration of the weight of nude mice, and the change of serum levels of LDH, ALT, Cr and WBC counts were examined and compared. The apoptotic rate of human cervical carcinoma HeLa cells xenografts was analyzed by FCM. The expressions of P53 and Bcl-2 proteins of HeLa cells xenografts were determined by Western blot-ting. Results:EVn-50 and its fractionated extracts could significantly suppress the increasing volume and weight of human cervical carcinoma HeLa cells xenografts in nude mice models in time-dependent manner, yet had no significant ef ect on the weight of nude mice, the serum levels of LDH, ALT, Cr and WBC were counted. When the xenografts were treated with EVn-50 and its fractionated extracts for 16 days, the apoptotic rate of xenografts cells were significantly increased, and the expression of P53 protein was up-regulated and protein level of Bcl-2 was decreased. Conclusion:EVn-50 and its fractionated extracts could suppress the growth of human cervical carcinoma HeLa cells xenografts in nude mice, which may be related to its pro-motion on xenografts cells apoptosis through down-regulation of Bcl-2 expression and activation of P53 expression.

Cellular chemokine CCL18increases matrix metalloproteinases expression and promotes ovarian cancer cell invasion and metastasis in vivo

Objective:To study the effect of cell chemokine (C-C motif) ligand 18 (CCL18) on ovarian cancer proliferation and metastasis.Methods:This study first analyzed the effects of overexpression of CCL18 on the growth in a subcutaneous ovarian cancer xenografts mouse model.Real-time quantitative PCR was used to detect the expression of matrix metalloproteinases (MMPs) in xenografts tissues.Then,an ovarian cancer orthotropic xenografts model was used to access the effect of CCL18 on ovarian cancer metastasis.Results:Over expressing CCL18 in SKOV3 cells did not significantly promote the tumor growth of subcutaneous xenografts.But the mRNA levels of MMP1,MMP7,MMP11 and MMP15 were significantly inncreased (P ＜ 0.05).The mRNA level of MMP12 was not changed (P ＞0.05).In orthotopic xenografts ovarian cancer mouse model,metastasis appeared in more organs in CCL 18 overexpressed SKOV3 cells than GFP/SKOV3 cells.Conclusion:CCL18 increased the expresston of MMPs in ovarian cancer cells and promoted metastasis of ovarian cancer cells in vivo.