Aim: To observe the apoptotic changes following exposure to EMP and to explore the possible injury mechanism in NIH - 3T3 fibroblasts. Methods: Following NIH - 3T3 cells were exposed to EMP,the proliferation and viability of NIH - 3T3 fibroblasts were estimated by hemacytometer and MTT Measurements. Apoptotic rate was measured by flow cytometer. The imnmohistochemical SP method was used to determine bcl- 2, p53. The positively stained cells were analyzed with CMIAS- Ⅱ image analysis system at a magnification 400. All data were analyzed by SPSS8.0 software. Results: The proliferation and viability of NIH- 3T3 cells were markedly inhibited and significant apoptosis was induced following exposure to EMP.EMP could increase the number of non- adherent cells, the highest ratio (33.9%) of non- adherent cells also occurred at 6h. The As70 value of MTT decreased immediately at 1 h, 6h following the cells were exposed as compared with the control (P ＜ 0.05). The highest ratio of apoptosis was 15.07% at 6h, then decreased gradually. Down - regulation of bcl - 2 and up - regulation of p53 were induced by EMP ( P ＜ 0.05). Conclusions: EMP could promote apoptosis of NIH - 3T3 fibroblasts. EMP could also down - regulate bcl - 2 level and up - regulate p53 level in NIH - 3T3 fibroblasts. Bcl - 2 and p53 gene may take part in the process of apoptosis.

核因子-κB 及 Bcl -2在胃癌组织中的表达及意义

目的：检测核因子-κB（NF -κB）及 Bcl -2在胃癌组织中的表达情况，探讨它们与胃癌临床病理特征的关系及两者之间的相关性。方法应用免疫组织化学方法（S - P 法）检测54例胃癌组织和32例正常胃黏膜组织中 NF -κB 及 Bcl -2的表达情况。结果胃癌组织中 NF -κB 及 Bcl -2的阳性表达率均明显高于正常胃黏膜组织（P <0.05）；胃癌组织中 NF -κB 的表达与肿瘤分化程度、癌组织浸润深度及临床分期有关（P <0.05）， Bcl -2的表达与临床分期有关（ P <0.05）；胃癌组织中 NF -κB 及 Bcl -2的表达具有关联性（ C =0.508，P <0.01）。结论 NF -κB 是重要的转录调控因子，Bcl -2高表达可引起细胞凋亡抑制，两者通过调节细胞凋亡而参与胃癌的发生发展过程，胃癌组织中 NF -κB 及 Bcl -2均呈现高表达且存在关联性，NF -κB 可能通过上调 Bcl -2的表达促进胃癌的发生发展。

三阴性年轻乳腺癌患者 BRCA1、TopoⅡα、Bcl -2表达和预后的关系

目的：探讨 BRCA1、TopoⅡα、Bcl -2在三阴性年轻乳腺癌患者中的表达情况和预后相关性。方法：回顾性分析62例年龄<40岁行手术切除乳腺癌患者临床资料，其中三阴性乳腺癌和非三阴性乳腺癌各31例。对其病理切片免疫组化 BRCA1、TopoⅡα、Bcl -2蛋白表达水平结果进行分析。结果：BRCA1、TopoⅡα、Bcl -2阳性和阴性在总生存率和无病生存率上比较差异有统计学意义（ P <0.05），三阴性乳腺癌患者的BRCA1阳性表达率明显低于非三阴性乳腺癌患者（P <0.05），而 TopoⅡα、Bcl -2两者比较差异无统计学意义（P >0.05）。结论：BRCA1、TopoⅡα、Bcl -2低表达和乳腺癌预后存在正相关，BRCA1低表达和非三阴性乳腺癌预后存在相关性。