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细胞因子诱导的杀伤细胞体外培养的初步研究
Objective To investigate the culture method of cytokines induced killer cells using CD3 monoclonal antibody,IL-2,IFN-γand IL-1αin vitro.Methods Peripheral blood mononuclear cells (PBMC) were collected by a blood cell separator from 8 patients with refractory lymphoma,then expanded by priming them with recombinant IFN-γ,monoclonal antibody (mAb) to CD3 and human recombinant IL-1α,followed by adding recombinant IL-2 the next day.The CIK cells were counted on d1,4,7,10,13 of incuba-tion,respectively.The phenotypic patterns were characterized before culture and on d 13 of culture.Results CIK cells colony formed on d3.On d13,the CIK cells counting reached up to (7-18) ×109 (mean 12.7 ×109 ),multiplying 44-140 times(mean 98 times).Cell survival rate was over 90%.The average per-centage of cells expressing CD3 +,CD4+,CD8 + and CD3 + CD56 + were also increased from (50.9 ±3.5)%,(29.9 ±1.7)%,(41.3 ±3.2)%,(1.6 ±0.2)% to (90.2 ±1.6)%,(40.6 ±5.5)%,(52.8 ±4.9)% and (33.1 ±4.0)%,respectively.Conclusions CIK cells developed by the culture method have high in vitro proliferate rate and tumor-killing capacity.The simplicity of the operation and autogenic source of the cells indicate the method could be applied clinically treating patients with refractory lymphoma .
