首页 > 文献资料
-
Objective: To study the vaccine potency of gene-modified tumor cells. Methods: The EL-4 lymphoma was transduced with recombinant retrovirus containing the murine GM-CSF gene or B7-1 gene. The effect of gene transduction on antitumor immunity was investigated. Results: Flow cytometry analysis showed that expression of their surface marker between wild-type EL-4 cells and gene transduced tumor cells was the same except for CD80 positive in B7-1 gene transduced cells. GM-CSF gene or B7-1 gene transduced EL-4 cells resulted in remarkable loss of tumorigenicity in syngenetic mice. The systemic protective immunity was induced against the challenge with EL-4/wt cells. Therapeutic vaccine with EL-4/GM-CSF or EL/7-1 cells could retard the growth of established early-stage EL-4/wt tumor significantly, but not retard the growth of late-stage EL-4/wt tumor. Irradiated GM-CSF gene transduced EL-4 cells showed strong vaccine effect against EL-4 cell challenge, but irradiated B7-1 gene transduced EL-4 cells showed weak vaccine effect. Remarkable cooperative antitumor effect against EL-4 cell challenge was observed when both irradiated EL-4/GM-CSF and EL-4/B7-1 were inoculated together. Conclusion: GM-CSF gene or B7-1 gene transduced combination of the two kinds of vaccine may have potential application value in human cancer treatment.
-
发展于西方的纠正“以瘦为美”的认知疗法是否有助于减缓非西方国家进食障碍患病率的增长？
进食障碍，这种威胁生命的疾病在西方国家很常见，直到近，进食障碍还被认为在非西方文化地区是罕见的。然而，进食障碍的患病率在很多相对富裕的非西方国家中迅速增长，因为这些地区的民众，特别是年轻的女性，在国际媒体的影响下，把“以瘦为美”这个已经被广泛传播的概念内在化了。这篇综述讨论了非西方地区中影响进食障碍发生的因素，特别关注了城市化、现代化、西方化和女性角色转变的影响。本文还介绍了在西方国家发展起来的、已经被证明可以成功消除“以瘦为美”的负面影响的认知失调纠正项目，并讨论了其在东亚和其它非西方国家中的应用价值。
