荧光定量PCR鉴别检测U.Urealyticum和U.Parvum方法的建立

目的:建立定量检测U.Urealyticum(UU)、U.Parvum(UP)的灵敏、特异的荧光定量PCR方法.方法:选择uu和UP的尿素酶基因设计两对引物和对应的探针组建2个TaqMan PCR反应体系,应用克隆的质粒建立系列标准品,作为模板建立荧光定量PCR标准曲线,并做重复性试验和特异性分析.结果:UU和uP两标准曲线具有良好的线性关系,相关系数分别为0.9895和0.9956,无非特异性扩增.结论:应用实时TaqMan PCR法定量检测UU、UP,具有快速、特异、灵敏、便捷的特点,为解脲脲原体感染的流行病学和病理机制的研究奠定了基础.

根除幽门螺杆菌治疗隆起糜烂性胃炎68例分析

目的 探讨根除幽门螺杆菌治疗隆起糜烂性胃炎的临床疗效.方法 对经电子胃镜及病理组织学检查确诊的68例成熟型疣状胃炎患者,男42例,女26例,年龄19～65岁,平均年龄36.8岁;HP阳性者59例(86.7%);给予口服瑞倍(350mg,2次/日),疗程4周;甲硝唑(400mg,3次/日),呋喃唑酮(100mg,3次/日),疗程10天:维生素E(60mg,3次/日),维生素AD(5万u,3次/日),疗程4周的抗HP为主的综合治疗.于用药4周后临床随诊并复查内镜及Hp.结果 68例中临床治愈46例(67.6%),好转18例(26.5%).无效4例(5.9%).59例Hp阳性患者治疗后Hp转阴50例(84.7%).并且发现Hp转阴的所有病例中治愈者有45例占90%.结论 本组疣状胃炎HP感染率达(86.7%),采用根除HP的综合治疗可获得满意的临床效果.

Objective To study the changes of baroreflex sensitivity (BRS) during head -up tilt test (HUT) in patients with vasovagal syncope (VS),and to examine the relationship between baroreflex sensitivity and neurohormonal factors. Furthermore, to investigate the effects of the changes of BRS on VS.Methods Forty- two patients with unexplained syncope (Among the 42 patients, there were 22 patients with positive HUT and 20 patients with negative HUT respectively) and 20 healthy volunteers (with negative HUT) underwent passive head-up tilt testing, Antecubital vein blood samples were taken before and after HUT, or at syncope. The fasting plasma endothelin ,serum nitric oxide (NO), serum NE were measured. The BRS was assessed on the basis of the linear regression slope the RR interval versus systolic arterial blood pressure during the increment in blood pressure after intravenous administration of phenylephrine. Results ( 1 )During the syncope, the BRS significantly reduced in HUT( + ) group than baseline. At the end of tilt, the level of plasma ET, serum NO in patients with positive HUT significantly increased compared with baseline or normal controls, and the plasma concentration of NE also had the trend of increase. ( 2 ) By multiple regression analysis, a significant negative correlation was found between baroreceptor sensitivity and the plasma ET, NO at the end of HUT in patients with positive HUT, but there was no relationship between BRS and NE. Conclusions During the syncope occure, the BRS in patients with VS decreased significantly compared with normal controls. The abnormal plasma ET, NO concentration might contribute to the mechanism of VS.