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慢病毒介导ACE2过表达对血管紧张素Ⅱ诱导的大鼠肝细胞的白蛋白表达及迁移能力的影响
目的：探讨血管紧张素转换酶2（Angiotensin-converting enzyme 2, ACE2）过表达对血管紧张素Ⅱ（Angiotensin II, AngⅡ）诱导的肝细胞白蛋白表达下调及细胞迁移性增强的抑制作用。方法常规培养大鼠肝细胞系BRL-3A细胞株，予以Ang II （10-7 mol/L）进行刺激，观察不同处理时间（24、48 h）组肝细胞内白蛋白、波形蛋白（vimentin）表达及细胞迁移性的改变；通过构建lentiACE2慢病毒载体，建立稳定过表达ACE2的细胞系，并分别用AngⅡ、A779进行干预，观察肝细胞内相应蛋白表达及其迁移性的改变。采用细胞免疫荧光、Western blot检测细胞中蛋白水平变化；Transwell迁移实验观察不同处理组细胞迁移能力的改变。结果 AngⅡ处理组肝细胞内vimentin表达显著增加、albumin表达减低，细胞迁移能力显著增强，并具有时间依赖效应。ACE2过表达大鼠肝细胞albumin表达明显升高，vimentin蛋白表达下降，该作用被MAS受体抑制剂A779阻断。同时ACE2过表达显著抑制AngⅡ的作用，vimentin合成显著下降，albumin表达水平显著升高，细胞迁移能力受到抑制。结论ACE2过表达可抑制AngⅡ诱导的肝细胞albumin表达下调及迁移能力增强。
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Clinical effects of phototherapy combined with albumin administration on hyperbilirubinemia in newborns
Objective To investigate the effects of phototherapy combined with albumin administration on hyperbilirubinemia in newborns.Methods Totally 64 newborns with neonatal hyperbilirubinemia were randomly divided into the control group and the study group,with 32 cases in each group.The newborns in control group received phototherapy and routine care,while those in study group were administrated with intravenous injection of albumin in addition to phototherapy and routine care.The concentration of total serum bilirubin,newborns'body weight and amount of milk intake were measured before and after phototherapy.Results There was a significant difference in the milk intake between two groups after phototherapy (P ＜ 0.05).There was no significant difference in the body weight or total serum bilirubin level of newborns between two groups after phototherapy (P ＞ 0.05).Conclusion Combined application of phothotherapy and albumin administration on neonatal hyperbilirubinemia could promote the newborns' appetite and improve the recovery of the neonates,but the effect on reducing blood bilirubin level and weight gain needs further study.
