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家蝇幼虫发育形态学用于死亡时间推断的基础研究
对死亡现场出现较多的尸食性昆虫的幼虫在各个恒温下的形态变化进行观察,旨在筛选出某些可用于判断幼虫生长时间的形态特征,并根据这些特征将幼虫期各生长阶段加以详细地划分,以满足实际工作中较精确地推断幼虫年龄和死后间隔时间的需求.本文就其家蝇部分报道如下.
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The Impact of Gender,Age and Tissues in vitro on Estimating Postmortem Interval by FTIR Spectroscopy
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Time-Dependent FTIR Spectral Changes in Rats of Massive Hemorrhage Death during the Later Postmortem Period
The aim of the current study was to investigate the spectra in the different organs of the rats which died of massive hemorrhage; to explore their spectral changes 15 days postmortem and the best mathematical model with different band absorption ratio changes to postmortem interval(PMD; and to compare the spectral changes of different temperature.Thirty male Sprague-Dawley rats were sacrificed by cutting abdominal aorta,and the cadavers were divided equally and kept at 4 ℃,20℃ and 30℃ in the control chamber.From the same rat,seven different organs were sampled at intervals of 1-15 days postmortem,and then measured by Fourier transfom infrared (FTIR) spectrometer.Six mathematical model functions were explored.The absorbance of bands and band absorbance ratios of absorption peak in each organ showed a time-dependent increase or decrease,most band absorbance ratios remaining stable for 7-15 days postmortem.Cubic model functions of the various bands absorbance ratios against PMI showed a stronger related coefficient.The absorbance bands with obvious changes at 20 ℃ showed stabilized tendencies at 4 ℃ and significant changes at 30 ℃ within 15 days postmortem.In addition,FTIR spectroscopy revealed a time-dependent metabolic process,with potential of being used to estimate PMI during 7 days postmortem,which merits further investigation.