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CDK2促进K562细胞早期红系分化
目的 探讨细胞周期调节蛋白CDK2对K562细胞红系分化的影响.方法 分别用CDK2表达质粒和干扰RNA分子转染K562细胞,用Western blot法检测过表达或干扰效率,使用real-time PCR和联苯胺染色法检测K562细胞分化.结果 CDK2在K562细胞红系分化早期呈现表达上升趋势;在K562细胞中过表达CDK2可促进hemin诱导的红系分化;反之,干扰K562内源的CDK2表达会对K562红系分化产生抑制作用.结论 CDK2在K562细胞早期红系分化过程中发挥促进作用.
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基于转录组学分析预测潜在的γ-和β-珠蛋白基因表达调控因子
目的 预测潜在的γ-和β-珠蛋白表达调控因子,为珠蛋白基因表达调控研究提供新的思路.方法 从GEO数据库中下载γ-和β-珠蛋白基因差异表达相关的两组RNA-seq数据,利用分化各时期共同存在的不同组织来源细胞间的差异表达基因及其预测出的上游调控因子,通过STRING数据库、Centiscape软件进行潜在调控因子的预测.结果 除预测到BCL11A、NF-E2、YY1和GATA1等已知的γ-和β-珠蛋白基因表达调控因子外,从两组数据共同预测出14个潜在调控因子.结论 分析预测出的包括MAPK1、TNF和IFNG等潜在的调控因子可能调控γ-和β-珠蛋白基因表达,需要近一步的分子生物学实验证实.
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MicroRNAs对胎儿血红蛋白表达的调控作用
MicroRNAs (miRs) play an important role in regulating diverse cellular processes.It has been reported that miRs are associated with the formation and maturation of erythrocytes, and the expression of globin genes at post-transcriptional level.Compared with normal human enrythrocytes, various miRs are altered in the patients with thalassemia.These changes also happen in the patients with diverse clinical manifestations.In this paper, we systematically summarized the recent progress about the expression dysregulation of miRs in β-thalassemia and their roles in regulating the levels of γ-globin and fetal hemoglobin.During β-like globin gene expression, miRs directly or indirectly regulate the levels of erythroid-specific transcription factors through post-transcriptional action, such as B-cell lymphoma 11A (BCL11A), myeloblastosis oncogene (MYB), specificity protein 1 (Sp1), Kruppel-like factor 3 (KLF3) and GATA1.These effects subsequently regulate the switch between γ-and β-globin gene expression and affect fetal hemoglobin production.Targeting miRs might be a novel therapeutic strategy for β-thalassmeia.
