外科手术患者血中细菌DNA的检出率

目的建立偶氮显色鲎试验并测定健康人和临床病人外周静脉血内毒素.方法利用偶氮显色法原理建立检测微量内毒素技术,测定50例健康成人及51例不同创伤病人外周静脉血的内毒素并对测定方法进行探讨.结果1.此法检测内毒素可达微量(10-2Eu/ml),自身变异系数为2.29%(n=5).2.健康成人外周静脉血内毒素正常值为<0.0330Eu/ml,与国际水准一致.3.病人外周静脉血内毒素水平与创伤程度有关,其血浓度随着创伤程度增加而增加,两者之间呈正相关,R2=0.667.结论本法灵敏度高,重复性好并可精确定量.

偶氮显色鲎试验定量测定人体外周静脉血微量内毒素的研究

目的建立偶氮显色鲎试验并测定健康人和临床病人外周静脉血内毒素.方法利用偶氮显色法原理建立检测微量内毒素技术,测定50例健康成人及51例不同创伤病人外周静脉血的内毒素并对测定方法进行探讨.结果1.此法检测内毒素可达微量(10-2Eu/ml),自身变异系数为2.29%(n=5).2.健康成人外周静脉血内毒素正常值为<0.0330Eu/ml,与国际水准一致.3.病人外周静脉血内毒素水平与创伤程度有关,其血浓度随着创伤程度增加而增加,两者之间呈正相关,R2=0.667.结论本法灵敏度高,重复性好并可精确定量.

We retrospectively analyzed the clinical data of 32 patients with medically intractable idiopathic Parkinson’s disease who had undergone staged bilateral deep brain stimulation of the subtha-lamic nuclei from January 2007 to May 2011. The vascularture of the patients who received two deep brain stimulations was detected using double-dose gadolinium-enhanced brain MRI. The dimensions of straight sinus, superior sagittal sinus, ipsilateral internal cerebral vein in the tha-lamic branch and ipsilateral anterior caudate vein were reduced. These ifndings demonstrate that bilateral deep brain stimulation of the subthalamic nuclei affects cerebral venous blood lfow.

静脉采血拔针后五种不同按压方法的应用分析与改进

静脉采血法是临床上常用的技术操作,但拔针后如何按压采血部位,使患者既能减轻疼痛,又能大限度减少瘀血青紫的不良后果,是值得探讨的.笔者通过对1989～2009年的中文核心期刊检索,共检索出42条关于"静脉采血拔针后压迫方法的探讨与研究"类文献,归纳出5种方法分别为屈肘棉签按压法、伸肘棉签横压法、伸肘三指棉签平行指压法、伸肘左右手拇指棉签按压法、伸肘大鱼际棉签按压法,并对5种方法在批量体检真空静脉采血拔针后按压中进行了应用.通过应用观察,各种不同按压对比或量化方法,尽管探讨研究结果有所不同,各自有其相应优缺点,但5种按压方法都能达到止血目的,只是对告知按压过程中影响止血效果的因素考虑不足,导致几种研究中都有一定的比例出现瘀血青紫现象.

真空采血致大面积皮下瘀血原因分析及预防

真空负压静脉采集血标本是近年来采用的一种新方法,此法简便易行,使医患双方都免受血液污染,防止了交叉感染[1],但易造成大面积皮下瘀血,给病人带来身心伤害.因此,我院门诊注射室1999年5月-2000年5月对真空负压采血造成大面积皮下瘀血原因进行了分析,并提出了预防措施.

静脉采血止血带扎结时间探讨

静脉采血是常见的护理操作之一,一次采血成功可以减轻病人的痛苦,提高工作效率和病人满意率.本文通过1000例门诊病人血标本采集,发现止血带扎结时间,对采血成功率有一定的影响.现总结如下.

血压测量对静脉采血后止血效果的影响

由于人们的生活水平不断提高,保健的需求也与日俱增.做体检又与患病就诊的程序有很大的不同,是一种具有很强的连贯性,一般都在2 h～3 h完成整个体检过程.静脉采血在身体检查中是必不可少的操作.在体检过程中发现,测血压时体检者静脉穿刺部位出血.现分析如下.

介入法岩下窦静脉取血的插管体会

采用介入法岩下窦静脉取血测促肾上腺皮质激素(ACTH),是区分垂体来源或异位来源的库兴综合症(Cushing综合征)可靠的检查手段,其诊断的阳性率明显高于垂体影像学检查,为指导手术治疗提供重要的依据,是一项值得推广的技术[1].但由于该检测有创,价格昂贵及技术复杂,国内医院开展较少.我院介入放射科自2003年已采用该技术取血数10例,均圆满成功,并达到临床诊断目的.现将岩下窦静脉取血的插管经验及体会汇报如下.

QOG磁极化量子血疗仪改善脑梗死后神经功能缺损的效果

BACKGROUND:Magnetopolarization quantum blood QOG treatment means using auto- venous blood or other people's blood of same type 150 to 200 ml and transferring back to body after external anticoagulation.

系统性硬化病患者外周血CD4+T细胞CD40L DNA甲基化研究

[目的]探讨系统性硬化病(SSc)患者外周血CD4+T细胞中CD40L基因调控序列的甲基化状态.[方法]密度梯度离心法分离SSc患者(女16例,男10例)和健康对照组(女15例,男10例)外周血单一核细胞,磁珠分选CD4+T细胞,提取DNA,亚硫酸氢钠处理DNA,巢式PCR扩增CD40L基因调控序列片段(包括启动子和增强子),转化进入大肠杆菌,每个样本挑取8个克隆进行测序.[结果]亚硫酸盐基因组测序结果显示,健康女性CD40L基因调控序列一半为甲基化,一半为去甲基化,女性SSc患者CD40L基因启动子和增强子区域平均甲基化水平均显著低于女性健康对照(P值均＜ 0.01)；男性SSc患者和男性健康对照CD40L基因启动子和增强子区域几乎全部为去甲基化,两组平均甲基化水平差异无统计学意义(P值均＞0.05).[结论]女性SSc患者CD4+T细胞中失活的X染色体上CD40L调控序列低甲基化,可能是导致CD40L在女性SSc患者CD4+T细胞中过度表达的原因之一.

带状疱疹患者单一核细胞Toll样受体3和9的表达

目的 探讨Toll样受体TLR3和TLR9在带状疱疹发病机制中的作用.方法 采用流式细胞仪检测40例带状疱疹患者外周血单一核细胞(PBMC)上TLR3和TLR9的表达水平.16例正常人群为对照组.结果 带状疱疹患者PBMC上TLR3和TLR9的表达水平均明显高于正常人对照组(P＜0.01).带状疱疹患者PBMC上TIR3和TLR9的表达水平与发病时间存在负相关关系(r值分别为- 6.23,-5.88,P值均＜0.01),但与性别、年龄无相关性.带状疱疹患者PBMC上TLR3和TLR9表达在各年龄组间(＜ 40岁,40 ～ 50岁,＞50岁),差异具有统计学意义(F=3.410,P＜0.01),且＜40岁年龄组TLR3和TLR9的表达明显高于其他两组.结论 TLR3和TLR9可能参与带状疱疹发病.

The present study was undertaken to investigate the effect of human PMNs on the production of TNF-α by the human peripheral blood mononuclear cells (PBMCs) and to elucidate its tentative mechanism. Human PMNs and PBMCs were isolated from the venous blood of healthy donors by dextran sedimentation and density gradient centrifugation. In the presence of lipopolysaccharide (LPS), PMNs and PBMCs were cocultured at the ratio of 2:1 for 20 h and the concentration of TNF-α in the supernatant was measured by enzyme-linked immunosorbent assay. The binding rate of monocytes with the fluorescein isothiocyanate-labeled LPS (FITC-LPS) and the mean surface fluorescence intensity of monocytes were analyzed by flow cytometry. Results showed that PMNs were capable of inhibiting the TNF-α release from PBMCs (P＜0.05). PMNs suppressed the TNF-α release from PBMCs by 45% on average when PMNs and PBMCs cocultured at the ratio of 2:1. Paraformaldehyde-fixed PMNs still demonstrated the same inhibition (P＜0.05)，which proved that the inhibition was dependent on cell-to-cell contact and suggested that effector molecules responsible for this effect existed on the cell surface of PMNs. In the presence of PMNs, the binding rate of monocytes with the FITC-LPS and the mean surface fluorescence intensity of monocytes were not affected compared with PBMCs alone (P＞0.05). As incubation time was prolonged, the binding of FITC-LPS to monocytes increased (P＜0.05). Thus PMNs did not block the binding of LPS with monocytes. It was concluded that PMNs suppressed the TNF-α release from PBMCs via cell-to-cell interaction. In a cell-contact dependent manner, PMNs might interfere with the signal transduction pathway through which LPS activated PBMCs, thus attenuating the response of PBMCs to LPS and downregulating the TNF-α release.

中西药联合灌肠治疗盆腔静脉淤血综合征78例

静脉盆腔淤血综合征(Pelvic venous congestion syndrome,PVCS)是由于盆腔静脉或静脉丛曲张、淤血引起的慢性下腹疼痛、性交后疼痛、植物神经功能紊乱症候群.我院2001年7月～2002年12月用中药煎剂加用2%盐酸利多卡因保留灌肠治疗PVCS147例,取得较好疗效,现报道如下.