首页 > 文献资料
-
雷公藤甲素纳米载药系统的研究进展
This paper reviewed the study of triptolide-loaded nano delivery systems (NDOS) in our group during the past. It was investigated for the preparation, characterization, pharmacology and toxicology of solid lipid nanoparticles (SLN), microemulsion and polymeric nanoparticles. The results indicated that the NDS presented more powerful activity and a lower toxicity in comparison with other drug carrier.
-
雷公藤内酯醇对内毒素激活小鼠腹腔巨噬细胞分泌促炎症介质NO和IL-6的影响
Objective Tripterygium wilfordii Hook. f. has been used for centuries in traditional Chinese medicine to treat autoimmune disease associated with increased production of the pro-inflammatory cytokine. Triptolide( TP) is a compound originally purified from T. wilfordii Hook f. and it has potent anti- inflammatory and immunosuppressant activities. In this study, we investigated the effect of TP on secretion of NO and IL-6 in celiac macrophages ( MΦ) activated by lipopolysaccharide ( LPS) in Kunming mice. Methods Celiac MΦ of mice were separated, purified, and activated by LPS, then cultured in vitro with TP of different concentrations. The level of NO in cellular supematants was determined by Griess reagent, and that of IL-6 was determined by ELISA. Results We found that pro-inflammatory cytokine NO activity in MΦ induced by LPS was significantly inhibited by TP ( 10-3-10 μg/ml) from 4-24 h in a time and dose- dependent manner (P < 0. 01). The level of IL-6 in MΦ was significantly inhibited by TP (10-3-10 μg/ml) at 12 h in a dose-dependent manner (P <0. 01). Conclusions We demonstrated that TP can inhibit levels of NO and IL-6 in celiac MΦ of Kunming mice activated by LPS.
-
TRP对KA诱导的AD模型大鼠学习记忆的影响及其作用机制
目的:观察雷公藤甲素(Triptolide,TRP)对海人藻酸(Kainic acid,KA)海马内注射后大鼠学习记忆的影响及其作用机制.方法:采用Morris水迷宫筛选空间学习记忆能力正常的SD雄性大鼠90只(200～220g).将实验动物分成3组:右侧海马注射生理盐水后生理盐水灌胃对照组(NS+NS)、右侧海马注射海人藻酸后生理盐水灌胃干预组(KA+NS)、右侧海马注射海人藻酸后雷公藤甲素灌胃干预组(KA+TRP).动物存活1天,3天,5天,7天,14天,每个时间点6只,处死前分别于各相应时间点用Morris水迷宫检测各组动物空间位置记忆能力；免疫组织化学方法结合图像分析技术检测海马CA1区神经元COX-2的表达.结果:与NS组(NS+NS)比较,KA组(KA+NS)大鼠逃避潜伏期延长(P＜0.05),跨越原平台次数减少(P＜0.05)；海马CA1区的神经元COX-2表达升高(P＜0.05)；TRP组(TRP+KA)与KA组比较,大鼠的平均逃避潜伏期从第5天起缩短(P＜0.05),跨越原平台次数增多(P＜0.05),海马CA1区神经元COX-2表达在5天,7天时下调(P＜0.05).结论:KA海马内注射,可以导致大鼠学习记忆功能障碍及上调海马CA1区神经元COX-2表达；雷公藤甲素干预治疗,能够改善动物的学习和记忆能力,能抑制KA诱导的海马CA1区神经元COX-2的表达.
关键词： KA triptolide 学习和记忆 Morris水迷宫 海马 -
雷公藤甲素对Aβ诱导的AD模型大鼠血脑屏障的影响
目的：探讨雷公藤甲素（Triptolide，T10）对β淀粉样蛋白（β-amyloid，Aβ1-42）海马内注射后大鼠血脑屏障的影响。方法：筛选健康的wistar大鼠30只（200-220g）。将实验动物分为3组：右侧海马注射生理盐水后生理盐水腹腔注射对照组（NS＋NS）、右侧海马注射Aβ1-42后生理盐水腹腔注射模型组（Aβ＋NS）、右侧海马注射Aβ1-42后T10腹腔注射干预组（Aβ＋T10）。动物存活4w和8w，每个时间点15只。到相应的时间后取材，采用伊文氏蓝示踪法观测脑组织蓝染程度，免疫组织化学方法结合图像分析技术检测海马区微血管上紧密连接蛋白claudin-5与occludin的表达。结果：与NS＋NS组相比，Aβ＋NS组脑组织出现蓝染，血脑屏障通透性明显增加且claudin-5与occludin的表达下调，4w组表达下降比8w组明显；经T10治疗后，4w组与8w组模型动物血脑屏障通透性有所改善，claudin-5与occludin表达上调，且8w组尤为明显。结论：Aβ诱导的AD模型大鼠血脑屏障的高通透性与其紧密接蛋白claudin-5与occludin的表达下调有密切关联，雷公藤甲素干预治疗后，能改善血脑屏障的通透性且claudin-5与occludin表达上调。
