AIM:Atherosclerosis primarily involved systemic arteries .Luminal surface , a monolayer of endothelial cells , of artery directly exposes to blood and is susceptible to active substances in the blood .Exosomes contain significantly amount of proteins and RNAs .Ex-osomes can be good and bad for cells , depending on their component .Thus, exosomes may contribute to atherosclerosis by affecting endothelial cells .This study analyzed the relationship of exosome proteins and atherosclerosis .METHODS: Fifty-six patients and healthy subjects were recruited and divided into two comparisons:healthy subjects vs atherosclerosis ( HS vs AS) , and hypertension vs hypertension plus atherosclerosis ( HT vs HT+AS) .Serum exosomes were decoded by protein mass spectrometry .The protein profile and function were analyzed by gene ontology ( GO) .RESULTS:It was found that five child terms repeatedly appeared in “response to stimulus” and “immune system process” of BP of the two categories ( HS vs AS and AS vs HT+AS):“positive regulation of innate immune response”,“immune response-activating signal transduction”,”activation of innate immune response”,“innate immune re-sponse-activating signal transduction” and “innate immune response activating cell surface receptor signaling pathway ”.Two child terms repeatedly showed in “binding” of MF of the two categories:“antigen binding” and “enzyme binding”.Two proteins, PSMA6 and PSMA7, were repeatedly shown in the two categories .CONCLUSION:GO analysis was utilized for structure hierarchy “tree” to illustrate these proteins involved in various terms in BP , CC and MF.The PPI analysis supplied proteins which may play potentially im-portant roles in AS process .Innate immune system and blood coagulation pathway contribute to AS formation .The proteins, PSMA6, PSMA7 and Annexin A2, may can be the new target proteins for prevention and treatment of AS .

安佳欣胶囊对抑郁症模型大鼠基因功能类表达影响的初步研究

目的 研究安佳欣胶囊对抑郁症模型大鼠基因功能类表达的影响.方法 分析抑郁症大鼠模型组和安佳欣胶囊组各8例样本的基因表达谱数据,筛选差异表达基因.利用基因功能分类体系Gene Ontology中的生物学过程子树和细胞组分子树,寻找和分析显著聚集差异表达基因的复合功能类,从分子水平和基因功能模块水平探索安佳欣胶囊对抑郁症模型大鼠基因表达谱的影响.结果 筛选出330个差异表达基因,并进一步识别了8个差异表达基因功能模块,主要涉及糖代谢、蛋白转运、谷氨酰胺代谢、凋亡诱导和神经发生.通过文献证实进一步发现了差异功能模块中7个可能与抑郁症发病相关的基因(Pfkm、Gpxl、Stxla、Ninj2、Plp、Evl、Nrnl)在两组动物中差异表达.结论 安佳欣胶囊可能增加抑郁症模型大鼠的单胺递质合成和释放,改善神经保护和神经发生功能,这些改变可能与安佳欣胶囊的抗抑郁作用有关.

基于功能模块组织癌细胞系基因表达谱的关联规则

目的提出一种在功能模块化水平上组织大量基因表达谱关联规则的新方法.方法以高频出现在关联规则后件中的基因为基础分类关联规则,再选择显著聚集前件基因的GO基因功能类作为功能模块关联规则的前件,获得功能模块表达关联规则.结果根据NCI60数据,发现了一些与癌症相关的基因及相应的基因功能模块表达关联规则.结论利用功能模块表达关联规则可以有效地挖掘疾病基因表达谱.