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AIM To determine whether MAP can be detected in archival paraffin embedded full thickness samples ofintestinal tissue from patients in China with Crohn's disease (CD), ulcerative colitis (UC), and in controlsubjects (NIBD) having surgery for bowel cancer.METHODS Optimized procedures for the removal of paraffin, recovery of tissue and access to MAP DNA,followed by MAP-specific nested IS900 PCR. Confirmation of specific amplification by Southern blotting andDNA sequencing.RESULTS IS900 PCR positive tests identified MAP in 9 (69%) of 13 CD, 1 of 3 UC and 2 (14%) of 14NIBD in the presence of correctly reporting positive and negative sample and reagent control reactions. DNAsequence analysis of the 298bp IS900 PCR amplification product from MAP in 2 Chinese CD patientsdemonstrated 99% homology with the GenBank IS900 sequence accession number X16293.CONCLUSION Although larger numbers of Chinese samples need to be studied, these initial results areconsistent with an exposure of human populations in China to MAP, and an involvement of this pathogen inchronic inflammation of the intestine of the Crohn's disease type. The results are in agreement with similarpositive studies reported from China, from Western Europe and elsewhere.
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1例Infliximab靶向治疗克罗恩病病人的护理
克罗恩病(Crohn's disease,CD)是一种病因不明的胃肠道慢性肉芽肿性疾患,好发于小肠末端及邻近的右侧结肠,且从口腔至肛门各段消化道均可受累, 其特点为病变呈节段性分布,临床以腹痛、腹泻、腹部肿块、瘘管形成和肠梗阻为特点,可伴有全身多处损害.病人病程长,反复发作,重者迁延不愈,给病人带来了极大的痛苦[1].我院于2007年12月收治1例重症克罗恩病病人,经采用Infliximab靶向治疗及精心护理,病情很快好转出院.现将护理体会报告如下.
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克罗恩病的流行病学及外科治疗
克罗恩病(Crohn disease,CD)是一种不明原因的、可累及全消化道的慢性复发性肉芽肿性炎症.其在我国乃至全世界范围内的发病率尚不甚清晰,在外科治疗的许多问题上也没有达成共识.
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Crohn病外科治疗的适应证及术式
在涉及Cronh病(Crohn's disease,CD)的外科治疗时,必须有一个明确的概念:CD是一种易复发的疾患,不可能靠几次手术切除来根治,而需配合内科治疗.外科治疗目的主要在于缓解临床症状,减轻病痛,手术时要为日后可能复发,需再次手术留有余地.