尤昭玲中医安胎思路的构想与实践

国家妇科名中医尤昭玲教授基于中医传统安胎方法不完全适应现代临床的背景,构思中医“别类分步”安胎方案.该方案依据体外受精-胚胎移植(IVF-ET)、人工授精(AI)、药物助孕、自然受孕“四类”不同受孕方式的特点,分胚胎着床前与后“两步”,并膳药结合养胎安胎.着床前以健脾长膜为主,注意把控各类受孕方式的胚胎着床时期;着床后辨证安胎,以补肾固胎为主,注意饮食宜忌.其健脾长膜治法的运用是对《内经》“治瘘独取阳明”的继承与发展.

加味四妙勇安汤对CIA大鼠关节炎性病理损伤的作用研究

[目的]探讨加味四妙勇安汤对Ⅱ型胶原诱导性关节炎（CIA）大鼠关节炎指数（AI）、关节病理损伤的改善情况，对大鼠体内白介素（IL）-6，IL-17，肿瘤坏死因子-α（TNF-α）表达的影响。[方法]建立CIA模型，将造模成功的SD大鼠随机分为模型组、阳性药（来氟米特）组、中药高、中、低剂量组，设正常对照组，每组6只，干预治疗12周。每周评价每只鼠的AI值。12周后麻醉处死，取各组大鼠右踝关节，光镜下观察病理学变化。采用逆转录-聚合酶链反应（RT-PCR）法检测大鼠膝关节滑膜IL-6、IL-17、TNF-α的mRNA转录水平，酶联免疫吸附实验（ELISA）法检测血清IL-6、IL-17、TNF-α的蛋白表达水平。[结果]中药3种剂量组在6~12周时AI分值较模型组明显下降（P﹤0.05或P﹤0.01）；而且在8~10周还分别表现出较阳性组显著的改善（P﹤0.05或P﹤0.01）。光镜检测结果显示：中药中剂量组明显改善了CIA模型大鼠关节滑膜增生、炎性细胞浸润情况。细胞因子检测结果显示各治疗组IL-6、IL-17、TNF-α的mRNA及蛋白表达显著降低（P﹤0.05或P﹤0.01）。[结论]加味四妙勇安汤对CIA模型大鼠关节炎改善作用的分子机制，可能与下调炎性细胞因子IL-6、IL-17、TNF-α的表达相关。

防治禽流感病毒药物的研究概况

通过对禽流感病毒的研究,目前已开出来的抗禽流感病毒的药物主要有:神经氨酸酶抑制剂(neuraminidae Inhibitors,Nis)、离子通道抑制剂(ion channel inhivitor)、核苷类药物三氮唑核苷和流感病毒受体阻滞剂四大类,但由于受药物本身不良反应及剂型的影响以及病毒抗原性变异显著等因素,研究和开发安全高效廉价易得的药物已势在必行.

基于AI的口腔颌面肿瘤学教学模式改革的初步探索

目的:探索基于AI的口腔颌面肿瘤学教学模式及教学实施方法.方法:研究融入AI元素的口腔颌面肿瘤学的教学改革理念,总结基于AI的医学教学相关手段与教学方法.结果:建立了基于AI设计与应用的口腔颌面肿瘤学新的多媒体教学课件,实施具有AI特点的授课教学方式的改进,提出口腔颌面肿瘤学教学适应AI的观点与措施.结论:建立基于AI的教学模式是教学改革的重要方向.

凋亡抑制基因Survivin与鼻咽癌预后关系的研究

目的探讨凋亡抑制基因Survivin蛋白产物在鼻咽癌(NPC)组织中的表达情况与凋亡指数(AI)、临床特征及预后之间的关系.方法应用免疫组化技术S-P法及TUNEL法,对51例鼻咽癌组织标本进行凋亡抑制基因Survivin蛋白产物及凋亡指数检测.结果在鼻咽癌组织中Survivin蛋白产物表达阳性率为 82.4%,其中高表达占51%,AI平均为 2.6%,Survivin的表达与AI存在相关性且与NPC病人的预后密切相关(P< 0.05).结论 Survivin的过度表达是导致NPC凋亡减少的一个重要原因,对NPC发生、发展起一定作用,而且对NPC的预后有重要的价值.

原发性醛固酮增多症25例分析

原发性醛固酮增多症(原醛症)是由于肾上腺皮质肿瘤或增生,醛固酮分泌增多而引起的以高血压、低血钾为主要表现的疾病.

Objective To analyze the relationship between polymorphism at the Apolipoprotein AI (Apo AI) gene and the risk for coronary artery disease. Methods A total of 107 patients (mean age 56 ± 11 years) diagnosed as having stable angina pectoris (SAP) (23 cases), unstable angina pectoris (UAP)(23 cases) or myocardial infarction (MI) (61 cases)were prospectively evaluated. DNA was obtained from the 107 patients and 50 controls. In order to determine the Apo AI genotypes at two polymorphic sites (G/A at -75 bp, and C/T at +83 bp), DNA was PCR amplified and digested with MspI. Results The frequency of carriers of the rare allele at the - 75 bp site (M1-) was 0.49 in cases and 0.30 in controls ( P＜ 0.05). The frequencies of the M 1 - allele among patients with SAP, UAP, MI and controls were 0. 37 (vs. Controls, P ＞ 0.05), 0. 54 (vs. Controls, P ＜0.05), 0.52 (vs. Controls, P＜ 0.05) and 0.30, respectively. The frequencies for carriers of the rare allele at the + 83bp polymorphism (M2) were observed among patients with SAP (0.09, vs. Controls, P ＞0.05), UAP (0. 11, vs. Controls, P ＞ 0.05) or MI (0.12, vs. Controls, P ＞ 0.05) and controls (0. 12).There was an slightly increase in the frequency of the M1 - allele in patients with SAP to UAP or MI (0.37vs. 0.54 vs. 0.52; all P ＞ 0.05) and M1 polymorphism as a risk factor for CAD (OR=3.74, P ＜0.05). In the + 83bp polymorphism there was no difference in the allelelic frequencies in cases and controls (0. 11 vs. 0. 12; P ＞ 0.05). There was no significant difference in the frequency of the M2- allele in patients with SAP to UAP or MI (0.09 vs. 0.11 vs.0. 12; all P ＞ 0. 05) and M2 polymorphism not as a factor for CAD ( OR = 0. 80, P ＞ 0. 05) Plasma lipoprotein values in patients with the allele M1 - and M2 - had no different levels than those homozygous for the M1+ andM2+ (P＞ 0.05). Conclusion M1polymorphism (M1 - ) may be as a risk factor for CAD and M2 polymorphism (M2 - ) not as a factor for CAD in Chinese Xinjiang Uygur and Han population.