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Large and small doses of drugs for improving blood circulation and removing blood stasis were used in model rats to treat mild chronic hepatic damage induced by carbon tetrachloride (CCl4). The results show that large dose of Dang Gui (当归 Radix Angelicae Sinensis) and Dan Shen (丹参 Radix Salviae Miltiorrhizae) (drugs for regulating blood flow) and small dose of Yu Jin (郁金 Radix Curcumae) and Niu Xi (牛膝 Radix Achyranthis Bidentatae) (drugs for activating blood flow) can significantly elevate the activity of SOD (P<0.05) and/or lower the T/K ratio, markedly reduce the MDA content (P<0.05 or P<0.01) and significantly decrease the activities of ALT and AST (P<0.05 or P<0.01), demonstrating that these drugs are effective in combating oxygen free radicals (OFR) in chronic liver damage. On the contrary, large dose of Tu Bie Chong (土鳖虫 Eupolyphaga seu Steleophaga) and E Zhu (莪术 Rhizoma Curcumae) (drugs for removing blood stasis) tend to increase the ALT and AST (P<0.05) activities. The results suggest that the synergism of elevation of the SOD activity and reduction of T/K ratio contributes to the action of drugs for improving blood circulation and removing blood stasis in combating the liver damage induced by CCl4.
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银杏叶提取物抗大鼠肝纤维化的作用
目的:探讨银杏叶提取物对实验性大鼠肝纤维化的预防及治疗作用机制.方法:采用四氯化碳腹腔注射诱导的大鼠肝纤维化模型,60只Wistar大鼠分为5组:模型组、银杏叶干预组、银杏叶治疗组、银杏叶组,另设正常对照组,应用HE染色观察大鼠肝组织的改变及Von-Gieson胶原纤维特殊染色观察肝纤维化程度,并采用免疫组织化学染色法及RT-PCR法检测肝组织Ⅰ型胶原、TGF-β1蛋白和mRNA的表达.结果:银杏叶干预及治疗组与模型组相比肝组织结构明显改善、纤维化增生程度减轻;肝组织内Ⅰ型胶原、TGF-β1的含量(Ⅰ型胶原:0.2 563±0.0 009 vs 0.2 885±0.0 025,0.2 541±0.0 076 vs 0.2 885±0.0 025,P<0.01;TGF-β1:0.2 785±0.0 012 vs 0.3 015±0.0 012,0.2 791±0.0016 vs 0.3 015±0.0 012,P<0.01)以及mRNA的表达均明显低于模型组(Ⅰ型胶原:0.0 778±0.054 vs 0.2 361±0.113,0.1 075±0.007 vs 0.2 361±0.113,P<0.01;0.523±0.015 vs 0.956±0.049,0.524±0.009 vs0.956±0.049,P<0.01);银杏叶组与正常对照组之间无差异.结论:银杏叶提取物可抑制肝星状细胞激活和转化,下调Ⅰ型胶原、TGF-β1蛋白质及其mRNA的表达,从而抑制或逆转纤维化形成.
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重组人肝再生增强因子对小鼠CCl4中毒性肝损伤的治疗作用
目的:利用大肠杆菌的基因工程技术,制备重组的人肝再生增强因子(ALR)药物,对于CCl4肝损伤的小鼠模型进行治疗,评价重组的人肝再生增强因子在肝损伤治疗中的效果和价值.方法:应用大肠杆菌系统,表达、纯化重组人肝再生增强因子蛋白.利用四氯化碳法制备肝细胞损伤的动物模型,以重组的人肝再生增强因子药物进行治疗,以生理盐水治疗作为对照,比较治疗后血清ALT,AST水平的变化,评价重组的人肝再生增强因子对于肝损伤的治疗作用.结果:成功制备了重组的人肝再生增强因子药物,纯度在98%以上.成功制备了四氯化碳的小鼠肝损伤模型,经过重组的人肝再生增强因子药物治疗后,血清中ALT,AST水平都有显著的下降(ALT:991 U/L对2 134 U/L,P<0.01;AST:938 U/L对1873 U/L,P<0.01).结论:重组的人肝再生增强因子药物,有希望成为各种原因引起的肝细胞损伤的治疗药物.
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四氯化碳对SD大鼠淋巴细胞DNA的损伤
四氯化碳(carbon tetrachloride,CCl4),又名四氯甲烷,是典型的肝脏毒物[1].此外,国内外还有报道,CCl4长期作用可引起胃肠功能紊乱及肝、肾功能明显损害等症状,对心肌及生殖系统也有一定影响等[2~5].
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四氯化碳对SD大鼠淋巴细胞DNA的损伤
四氯化碳(carbon tetrachloride,CCl4),又名四氯甲烷,是典型的肝脏毒物[1].此外,国内外还有报道,CCl4长期作用可引起胃肠功能紊乱及肝、肾功能明显损害等症状,对心肌及生殖系统也有一定影响等[2~5].