附睾间质细胞肿瘤一例

患者男,42岁.左侧附睾头部肿物4年,无性欲丧失及男性乳腺发育等内分泌紊乱的症状.近2年逐渐增大.于2004年4月1日入院.B超示肿物为实性,精索、左侧睾丸、右侧睾丸、右侧附睾均未见异常.临床诊断:左附睾头部纤维瘤?结核?行外科手术切除.术中可见肿物位于左侧附睾头部,边界清楚,睾丸未见异常.

大鼠精索静脉曲张对Leydig细胞中睾酮、细胞凋亡和StAR mRNA的影响

SET is a multifunctional protein involved in regulating many biological processes of the cell cycle. It is also a regulator of steroidogenesis in the ovary. However, the expression of SET protein in testis, and its function, still remains ambiguous. In this study, we observed the expression of SET in the testes of mice at different developmental stages, and have discussed its potential function in regulating spermatogenesis and androgen production. Forty?eight male mice at different developmental stages(1week old as the infancy group; 4weeks old as the prepubertal group; 12weeks old as the adult group; over12months old as the ageing group) were used. Cellular location of SET protein in the testes was observed by immuno?histochemistry. Expression levels of Set mRNA and SET protein were analyzed by quantitative polymerase chain reaction and Western blotting. SET protein was expressed in spermatogonial cells and spermatocytes; the highest level was mainly in haploid and tetraploid cells of the prepubertal and adult groups, and Leydig cells of the adult and ageing groups. There was a low expression in Sertoli cells. Expression of Set mRNA in the prepubertal group was signiifcantly higher than that in the adult group(P<0.05), while expression of SET protein was at the highest level in the adult group(P<0.05).SET protein is mainly expressed in spermatogonial cells and spermatocytes, and poorly expressed in Sertoli cells, suggesting that it is involved in spermatogenesis. Expression of SET protein in Leydig cells suggests a possible role in steroidogenesis.

重组分泌型Atrn蛋白（rAtrn）对原代培养大鼠睾丸Leydig细胞功能的影响

目的：检测rAtrn对基础状态和hCG刺激下体外培养大鼠睾丸Leydig 细胞睾酮分泌的影响，进一步阐明Atrn在生殖内分泌中的作用。方法：分别用0、500、1000、2000、4000、8000 ng/ml rAtrn对基础状态和10ng/ml hCG刺激下的原代培养大鼠睾丸Leydig 细胞进行干预，分别于干预24h、48h收集培养液检测睾酮含量。结果：基础状态和hCG刺激下，不同浓度rAtrn干预24h、48h后，大鼠 Leydig细胞睾酮产量无显著性差异。结论：重组分泌型 Atrn在0～8000ng/ml 剂量范围内，均不能显著影响体外培养大鼠L ey dig 细胞生成睾酮的能力。