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AIM To investigate the effects of sinusoidal endothelial cell (SEC) conditioned medium on the expression ofconnective tissue growth factor (CTGF) in rat hepatic stellate cells (HSC).METHODS By in situ collagenase perfusion and two-step Percoll gradient centrifugation, SECs wereisolated and cultured from normally and CCl4-treated Wistar rats, and the SEC conditioned media werecollected. HSCs were prepared from Wistar rats by in situ perfusion and single-step Nycodenz gradient, andwere cultured with SEC conditioned media. Expression of CTGF in HSC was assessed using reversetranscription-polymerase chain reaction (RT-PCR).RESULTS Expression of CTGF was not found in freshly isolated HSC and in primary culture of HSC onday 4 with SEC conditioned media from normal rats, but was present in primary culture of HSC on day 4 withSEC conditioned media from CCl4-induced liver fibrosis rats. Expression of CTGF was observed in culture-activated HSCs, and the effect of SEC conditioned media from CCl4-induced liver fibrosis rats on theexpression of CTGF gene in activated HSCs was not significant.CONCLUSION Expression of CTGF might be relative to the activation of HSC and the liver fibrogenesis,and damaged SECs play a very important role in the early stage of activation of HSC.
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肝窦内皮细胞在肝纤维化形成中的作用
肝窦内皮细胞(liver sinusoidal endothelial cell,LSEC)是肝窦壁的主要组成细胞,其独特的结构、免疫表型及功能在肝脏的生理和病理过程中发挥重要作用;正常LSECs表面有窗孔,内皮下缺乏基底膜,是肝细胞与肝窦血液间物质交换的重要通道;慢性肝病中,LSECs失窗孔,内皮下出现基底膜,形成类似连续型毛细血管的结构,这一过程称为肝窦毛细血管化;肝窦毛细血管化的形成导致肝细胞损伤、细胞外基质(ECM)沉积、肝脏微循环障碍等一系列病变,促进肝纤维化、肝硬化的发生发展.LSECs独特的结构及重要的功能日益引起人们的兴趣,相关研究层出不穷,本文将结合近几年国内外相关研究进展,就LSECs的结构、功能特征及其在肝窦毛细血管化形成中的作用作一简要综述.
