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pSVPoMcat微基因修饰雪旺细胞脊髓内移植对损伤脊髓细胞的保护作用
Objective To study the protective effects of the intracord transplantation of microgene pSVPoMcat- genetically- modified Schwann cells (MSCs)on spinal cord injury (SCI).Method Rats with semi- division(SD) of the spinal cord was divided into 4 groups.Group S consisted of the rats with SD treated with the transplantation of MSCs, Group B of the rats with SD treated with the transplantation of SCs without genetic modification,Group C of the rats with SD without treatment and Group D was the normal control. 8 hours after operation,the half of the rats of each group were killed and the injured segment of the spinal cord was resected to be examined with atomic absorption spectrophotometry . Another half of the rats of all the groups were examined with neurological function tests to have a combined behavioral score (CBS).Result There was a significant increase of water content and Na+ and Ca2+ ions and a decrease of K+ and Mg 2+ ions in the injured cord segment of Group C and a statistically significant recovery was observed in Group A. The intracord transplantation of pSVPoMcat genetically modidied SCs improved the neurological outcome of spinal cord injury.Conclusion Our findings indicate that intracord transplantation of pSVPoMcat- genetically- modified- Schwanncells exerts protective effects on the injured segment of the spinal cord through the improvement of the internal ion environment of the spinal cord.
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大中型手术期间应用乌司他丁对机体保护作用的临床研究
近些年,国外逐渐将尿蛋白酶抑制剂--乌司他丁(ulinastatin,UTI)用于围术期抗手术侵袭及防御组织、器官的损伤,并取得了良好的效果;而目前国内关于这方面的临床研究较少,本文通过检测粒细胞弹性蛋白酶、纤维连接蛋白、肝肾功能及血液流变学等的变化,较为全面地评估围术期应用UTI对机体的保护作用.
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植物次生物质及其防御昆虫的研究进展
昆虫是动物种类多的类群,约占动物种数的四分之三.昆虫以其体形小、善飞、行动方便、食量少、繁殖周期短等优势繁衍后代并生生不息.昆虫从植物中获取食物,对植物造成损伤,同时对植物造成自然选择压力,使它产生化学和物理防卫机制,并产生次生物质对昆虫起防御作用.次生物质的名词是由Kossel和Czapek在上世纪20年代首先提出来,是复杂的分支代谢途径的后产物,不直接参与维持产生者的生长发育和生殖有关的原始生化活动,但有的与基础代谢物质也没有绝对界限.目前,国内外学者就植物次生物质与昆虫的相互作用做了多方面的研究,本文对其研究进展作一综述.
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沙眼衣原体泌尿生殖道慢性持续感染患者人白细胞抗原DQA1基因多态性研究
目的 探讨人白细胞抗原DQA1 (HLA-DQA1)等位基因多态性与沙眼衣原体泌尿生殖道慢性持续性感染的相关性.方法 PCR和基因测序方法,对80例沙眼衣原体泌尿生殖道慢性持续感染患者、80例沙眼衣原体泌尿生殖道一般感染患者及80例正常人的HLA-DQA1等位基因进行检测.结果 HLA-DQA1*0102和DQA1*0501在沙眼衣原体泌尿生殖道慢性持续感染患者中的基因频率分别为22.5%、5.0%,在一般感染组的基因频率分别为5%、20%,而在正常人对照组的基因频率分别为2.5%、17.5%.沙眼衣原体泌尿生殖道慢性持续感染患者的HLA-DQA1*0102等位基因较一般感染组及正常人对照组增高,差异有统计学意义(x2=14.6286,P<0.01);而HLA-DQA1 *0501等位基因在持续感染患者中下降,差异有统计学意义(x2=6.2598,P<0.05).结论 HLA-DQA1 *0102可能是沙眼衣原体泌尿生殖道慢性持续感染的易感基因或与易感基因相连锁.HLA-DQA1*0501等位基因可能具有阻止发生沙眼衣原体泌尿生殖道慢性持续感染的作用.
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对辐射防护效果评价方法的探讨
对医用放射设备和工业射线探伤设备的防护工程进行防护效果的监测,评价时,经常遇到因监测方法和评价指标不同而发生防护效果评价方面的争议,造成一些不必要的矛盾和浪费.
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腺苷对心肌梗死再灌注无复流的保护作用及其对一氧化氮和内皮素影响的实验研究
冠状动脉无复流是指闭塞的冠状动脉完全再通后,在没有栓塞、痉挛、夹层等机械性梗阻证据存在的情况下,血流不能充分灌注冠状动脉远端血管的一种现象[1].冠状动脉无复流的存在既是心肌损伤、梗死延展的标志,也是心室重构、心功能障碍的预测指标[2~7].
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BACKGROUND:Cytochrome P450 ( CYP) epoxygenases metabolize arachidonic acids ( AA) to form epoxyeicosatrienoic acids
(EETs), which exert beneficial roles in the treatment of cardiovascular diseases , but little is known about its role on adventitial remo-deling.METHODS:We used C57BL/6J mice in vivo and primary rat adventitial fibroblasts ( AFs) in vitro treated with angiotensin II (Ang II) to investigate the effects of CYP2J2 gene delivery and exogenous EETs administration on adventitial remodeling .RESULTS:CYP/sEH system was found to exist in human adventitia , and involved in adventitial remodeling process .Exogenous EETs administra-tion significantly inhibited Ang II-induced AFs activation , characterized by differentiation , proliferation, migration, and collagen syn-thesis.These protective effects were partially reversed by PPARγantagonist GW9662 pretreatment or SOCS3 siRNA transfection.EETs suppressed Ang II-induced IκBαphosphorylation , subsequent NF-κB nuclear translocation via PPARγdependent signaling pathway in AFs.Additionally, EETs reduced Ang II-induced JAK2, STAT3 phosphorylation and subsequent phosphor-STAT3 nuclear transloca-tion, which were mediated by SOCS3 induction but independent of PPARγactivation.Furthermore, rAAV-CYP2J2 gene delivery re-duced vessel wall thickening , AFs differentiation , proliferation and collagen deposition in aortic adventitia induced by Ang II infusion , which were mediated by NF-κB and SOCS3/JAK/STAT signaling pathways in blood pressure-dependent and -independent manners , re-spectively.CONCLUSION:We concluded that CYP2J2 overexpression attenuated Ang II-induced adventitial remodeling via PPARγ-dependent NF-κB and PPARγ-independent SOCS 3/JAK/STAT inflammatory signaling pathways .