人类羊水源类ES细胞多能性干细胞的分离培养

Stem cells are classified into embryonic stem (ES) cells and adult stem cells, which are the progenitors of any cell types of the body or tissues, and have the ability to self-renew and produce one or more differentiated cell types.

羊水栓塞的简易病理诊断

羊水栓塞是产科危险的并发症之一,死亡率高达70%～85%.该病起病急剧,发展迅速,后果严重,诊断标准常以临床诊断作为推测[1].病理诊断因介绍的方法难以操作,国内医院少有应用.如何提高病理在诊断中的作用,推广简便易行的方法,我们在近年的工作及接受外院送检标本的过程中取得了一些经验,现报道如下.

羊水来源干细胞与心肌组织再生性治疗的研究进展

再生性治疗是修复受损心肌的一种治疗策略,其主要内容是将细胞移植入受损心肌,新的细胞辅助修复并恢复心功能.Müller-Ehmsen等[1]将胎鼠或新生乳鼠心肌细胞移植到大鼠心肌梗死模型中,观察到移植心肌细胞在梗死区存活,梗死心肌厚度增加,左心室功能得到改善.但受伦理学及供体数量限制,这种方法不可能应用于临床.近十年,干细胞被广泛关注,就在于实验模型和初步临床研究均提示受损心肌组织可以通过干细胞移植达到功能恢复.

“在产前诊断中应用羊水病毒PCR技术的时机选择”点评

1原文摘要Objectives To determine which prenatal ultrasound findings indicate the need to also obtain PCR studies for viral genome in women undergoing midtrimester amniocentesis.Methods This was a retrospective observational study on women that underwent amniotic fluid karyotyping and viral PCR testing for history or ultrasound based indication.Amniotic fluid was tested for adenovirus,cytomegalovirus,respiratory syncytial virus,enterovirus,Epstein-Barr virus,and parvovirus B19 using multiplex PCR study with multiple appropriate controls.Ultrasound findings were coded as normal or abnormal with 34 categories of ultrasound abnormality stratified into 18 subgroups.

特发性婴儿动脉钙化症一例

SE REPORTA female infant, 30 min after birth, was transferred to neonatal intensive care unit (NICU) from the Department of Obstetrics on October 7th, 2006 because of anasarca and no spontaneous breath. The infant was born to her mother at gravida 3, para 3, and her gestational age was 34 weeks. She was delivered by cesarean section because of intrauterine distress and polyhydramnios. Prenatal color Doppler flow imaging ( CDFI ) suggested bilateral hydronephrosis and ascites. The Apgar scores were 3 at 1 min and 7 at 5 min. Amniotic fluid was clear but excessive. The infant had anasarca, cyanosis and had no spontaneous breath at birth. She was admitted to the NICU after tracheal intubation.

羊水胎粪污染新生儿348例头颅B超筛查结果分析

羊水胎粪污染(Meconium Staining of the amniotic fluid,MSAF)在产科很常见,对围生儿除易引起胎粪吸入、新生儿窒息外,对颅脑损伤的影响不可忽视.本院儿科自1996年4月至1999年3月,对凡在本院出生的羊水Ⅱ～Ⅲ度粪污染新生儿全部行头颅B超筛查.对其中无明显窒息症状及体征者,将B超结果报告如下:

羊水量异常的超声诊断及临床意义

所谓羊水,是指妊娠时子宫羊膜腔内的液体.羊水中98%～99%是水,1%～2%是溶质.溶质一半是有机物,一半是无机盐,此外还有极少量的细胞.在整个妊娠过程中,它是维持胎儿生命所不可缺少的重要成分.

羊水栓塞病人心肺功能变化

羊水栓塞是由于羊水物质进入母体循环,而引起的严重的综合征,典型表现为突发性低血压,低血氧及凝血功能障碍,但临床表现有很大差异,可在短期内猝死,也有仅以凝血功能障碍为主要表现者.

羊水栓塞诊断治疗的新进展

羊水栓塞(amniotic fluid embolism,AFE)是一种极为凶险的产科并发症.AFE已上升为产科死因的首位[1].近几年来对AFE的发病机制已有了更新的认识,现就AFE的诊断、治疗新进展进行讨论.

206例羊水指数异常孕妇围生儿预后分析

随着围产医学的发展,对妊娠期羊水过少的认识逐渐深入,羊水过少是引起围生儿死亡的主要原因之一[1].该文回顾性分析崇阳县人民医院2005年6月至2007年12月206例羊水指数(AFI)异常孕妇的临床资料,探讨围生儿预后的相关影响因素.

The dynamic level of immunosuppressants such as alpha-fetoprotein(α-FP), gestation associated protein, progesterone, estradiol, chorionic gonadotropin, etc. of peripheral sera and the regulatory mechanism of cellular immunity in the peripartum stages in sows were determined by advanced radioimmunoassay (RIA) and immunochemistry methods in this study. The effects on lymphocyte cultivated in vitro by the immunosuppressants mentioned above were tested by cell culture technology, respectively. The results were as follows: (1) α-FP: The level of α-FP of peripheral sera in the stage of ante-parturition was slightly higher than that of post-parturition in sows, but it didn't show statistical difference. The concentration of α-FP of peripheral sera in conceptus sows had no obvious effect on lymphocyte cultivated in vitro, and it indicated that α-FP was not the major immunosuppressant in the pregnancy of sows. However, the α-FP, whose concentration was equal to that of amniotic fluid, had obvious inhibition on lymphocyte cultivated in vitro. (2) Gestation associated protein: The level of gestation associated protein was maintained highly (100-162 mg/L) in peripheral sera and amniotic fluid in the stage of ante-parturition in sows, then descended gradually after parturition and disappeared step by step 5-10 d after parturition. The gestation associated protein, whose concentration were equal to that of peripheral sera and amniotic fluid, were added to lymphocyte culture in vitro, and the effect on lymphocyte activity was not observed yet. This showed that gestation associated protein was not the major immunosuppressant in the peripartum stage in sows. (3) Estradiol and progesterone: Estradiol or progesterone at the level of sera in pregnant sows, could lower the rate of lymphocyte transformation and the level of cellular immunity. (4) Chorionic gonadotropin: The level of chorionic gonadotropin of peripheral sera in the stage of ante-parturition was slightly higher than that of post-parturition in sows, but it didn't have statistical difference. The chorionic gonadotropin, whose concentration was equal to that of sera, was not yet observed obvious effect on lymphocyte cultivated in vitro. (5) Added the five above-mentioned immunosuppressants to lymphocyte culture in vitro, whose concentration were equal to those of sera in pregnant sows, they could drop the rate of lymphocyte resette formation and lymphocyte transformation, lower the level of cellular immunity and reduce the concentration of IL-2 which synthesized and secreted by lymphocyte in culture remarkably. Their inhibitory intensity of cellular immunity was evidently higher than that of any single immunosuppressant.

迟发性羊水栓塞的临床特征和治疗

羊水栓塞(amniotic fluid embolism,AFE)是指分娩过程中羊水进入母体循环而引起的肺栓塞、弥漫性血管内凝血(DIC)、肾衰竭等一系列病理改变的严重综合征,是孕产妇死亡的重要原因之一[1].典型症状是呼吸困难、发绀、出血和昏迷.少数患者在阴道分娩或剖宫产后1h内,不经心肺功能衰竭及肺水肿阶段直接进入凝血功能障碍所致的大量阴道出血或伤口渗血阶段,为迟发型羊水栓塞(delayed amniotic fluid embolism,DAFE)[2].DArE因缺乏急性AFE典型的临床表现,容易误诊而延误治疗.本文就我院2001年1月至2011年12月11年来诊断的DArE进行回顾性分析,探讨其临床特征和早期诊断和治疗方法.