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与克拉霉素耐药相关的幽门螺杆菌基因23S rRNA序列分析
幽门螺杆菌是寄生于人体胃部的革兰阴性杆菌,是胃溃疡、十二指肠球部溃疡和胃癌的主要致病因素之一,与胃黏膜相关淋巴组织淋巴瘤关系密切.随着大环内酯类抗生素广泛应用于治疗幽门螺杆菌感染,幽门螺杆菌的耐药现象日益严重.现已证实,幽门螺杆菌对克拉霉素耐药主要与其23S rRNA基因的点突变有关.为此,本研究通过对江苏苏中沿海地区分离培养的幽门螺杆菌菌株的23S rRNA部分基因片段进行序列分析,希望了解本地区幽门螺杆菌耐药菌株的基因突变和耐药的关系.
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不同心脏激动顺序下心肌力学与血流动力学状态及超声研究进展
随着心脏功能研究的不断深入,心脏不同激动顺序下心肌力学及血流动力学状态改变越来越受到临床心脏病学家的重视.现有的各种心脏起搏模式常常引起心脏激动顺序紊乱,导致异常的心肌力学及血流动力学效应.如何精确评价不同激动顺序下的血流动力学和心肌力学改变及其关联关系,为选择更为生理性的心脏起搏方式提供依据,已成为目前研究的热点.磁共振成像(MRI)虽然可精确和重复地分析室壁三维运动和功能,但价格昂贵、耗时过长及帧频较低限制其在临床工作中的应用.迅速发展的超声心动图为进行心脏心肌力学及血流动力学评价提供了新的技术手段.
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犬小孢子菌膜蛋白PQ-LRP基因全长cDNA的克隆
目的 克隆犬小孢子菌膜蛋白PQ-LRP(PQ-loop repeat protein)基因全长cDNA,探讨在头癣发病机制中的作用.方法 选用犬小孢子菌头癣株(A518)为实验株,采用cDNA快速末端扩增法(RACE),克隆PQ-LRP基因的全长序列.结合生物信息学方法对获得的序列进行初步功能分析.结果 获得犬小孢子菌PQ-LRP全长序列为1522 bp,拥有一个1080 bp的开放阅读框,编码359个氨基酸,5 '非编码区为49 bp,3 '非编码区为393 bp;同源性比对与断发毛癣菌的PQ-LRP同源性达到81%,与红色毛癣菌PQ-LRP同源性达到79%.结论 克隆出犬小孢子菌膜蛋白PQ-LRP cDNA全长序列,为研究膜蛋白PQ-LRP基因在犬小孢子菌病中的功能奠定基础.
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AIM: PSP94 has been shown promise as a potential prostate cancer marker and it was reported that PSP94 can inhibit the growth of prostate cancer cell in vitro and in vivo. This study aimed to construct recombinant human PSP94 expression vector. METHODS:The PSP94 cDNA was obtained from normal prostate tissue, and recombinant plasmid pUC19-PSP94 was constructed. The target gene was identified and sequenced. Then the PSP94 gene was inserted to the secretory expression vector. RESULTS:The gene sequence of PSP94 was identified. The recombinant vector was constructed. The secreted PSP94 was isolated and identified by Western blot. CONCLUSION:The recombinated PSP94 could expressed PSP94 successfully.
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广西狂犬病毒野毒株的基因和抗原分析(英文)
Analyse the genetic and antigenic variant of rabies virus in Guangxi with epidemiological method, monoclonal antibodies reaction pattern and molecular biological technique. Rabies incidence has increase in recent three years in Guangxi, especially in mountainous areas. Investigation into the human rabies found that there are about 20% vaccinated defeat cases.On the basis of their reactivity to monoclonal antibodies against the viral nucleocapsid protein(mAb-N),4 street strains isolated from different areas where enzootic rabies is shown had different reaction patterns compared with Chinese vaccine strain(3aG). The nucleotide and amino acid sequence of street strain(GX89_1) G and N gene were compared with 3aG, the overall nucleotide homology of GX89_1 with 3aG was 84.5% and amino acid homology of GX89_1 with 3aG was 89.5% in G gene.The overall nucleotide homology of GX89_1 with 3aG was 86% in N gene and they were not in the same group.There are several amino acid replacement in AA 243_268 of G protein that can affect the antigenicity of rabies virus.The findings suggest that there are different street strains of rabies virus in Guangxi.
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pGEX-e23 (scFv) PE40融合基因的构建及其在大肠杆菌中的表达
目的 在大肠杆菌中表达抗原癌基因c-erbB-2表达产物p185的单 链抗体e23(scFv)/假单孢菌属外毒素活性片 段PE40免疫毒素的融合蛋白,为乳腺癌、胃癌等多种c-erbB-2呈过量表达的恶性肿瘤的免疫治疗奠定基础. 方法 去除克隆在真核表达载体pLNCX中的e23 (scFv) PE40基因5′端编码信号肽的核苷酸序列,并将改建后的融合基因克隆到原核融合表达载体pGEX-4T中表达. 结果 序列测 定 表明. 改建后的抗p185 e23(scFv)/PE40序列正确. 融合基因经IPTG诱导表达4 h后,经SDS -聚丙烯酰胺凝胶电泳分析,在Mr 90 000处出现一条新生蛋白带,表达量约占菌体总蛋白的15%. 结论 成功改建并在原核中表达了抗p185 e23 (scFv)/ PE40融合蛋白.
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关键词: sequence of
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Objective: To investigate the effect of fetal spinal cord (FSC) graft with different methods on axonal pathology and neurological function recovery after spinal cord injury (SCI). Methods: Forty Wistar rats were divided into 4 groups. In Group A, the spinal cord was injured and hemisected. In Group B, fetal spinal cord (FSC) was transferred into the injured site. In Group C, after having done as Group B, the upper and lower spinal nerve roots were anastomosed. And in Group D, after having done as Group B, the pedicled omentum was transferred into the hemisection cavity. At 6 weeks after operation, light and electronic microscopes were used to examine the axonal pathology. The neurological function was assessed with inclined plane tests in the open field. The number of axons was quantitated by a computer image analysis system. Results: A greater loss of axons was observed in Group A than that of other groups at 6 weeks. The sequence of the reduced rate of the axons was as following, Group A>Group B>Group C>Group D (P<0.05). The remaining axons were paralleled with the significant improvement in neurological function recovery of the rats. Conclusions: It indicates that FSC and pedicled omentum grafts after SCI can protect the axons and promote the neurological function recovery of the rats.