首页 > 文献资料
-
Helicobacter pylori infection is associated with increased expression of macrophage migration inhibitory factor by T cells and macrophages in gastric mucosa
AIM Macrophage migration inhibitory factor (MIF) plays a pivotal role in inflammatory/immune diseases.This study aimed to determine MIF expression in H.pylori-induced gastritis,and the effect of H.pylori on MIF expression in monocytes in vitro.METHODS Seventy-nine patients (M/F,39/40,mean age,52 yrs) referred for upper endoscopy were selected;19 with gastric ulcer,15 with duodenal ulcer and 45 with non-ulcer dyspepsia (NUD).Gastric antral and body biopsies were obtained for histological examinations,double immunostaining for MIF/T-cells (CD45RO) and MIF/macrophage (KP1),and in situ hybridization for the expression of MIF mRNA.THp-1,a monocyte cell line,was co-incubated with different concentrations of the whole cell proteins prepared from H.pylori strain ATCC26695 or its isogenic type with cagA gene deleted.The expression of MIF protein was determined by using enzyme linked immunosorbent assay and the MIF mRNA by retrospective transcription-polymerase chain reaction techniques.RESULTS H.pylori was detected in 50 patients (10 with gastric ulcer, 15 with duodenal ulcer and 25 with NUD).Overall,the numbers of total T-cells,MIF+T-cells,total macrophages,MIF+macrophages and MIF mRNA+ cells were greater in the gastric antrum than in the body.There was a significant increase in the numbers of total T-cells, MIF+ T-cells,total macrophages,MIF+macrophages and MIF mRNA+cells in H. pylori positive,compared with H.pylori negative patients,in both the antral and body mucosa.Moreover,the cell numbers increased with more severe chronic gastritis in both the antrum and body.The numbers were also significantly higher in ulcer patients than in NUD patients, particularly in H. pylori positive patients.In vitro,the expression of MIF protein and mRNA in monocytes was significantly increased by incubation with H.pylori whole cell proteins,in a time and dose dependent manner.CONCLUSIONS H.pylori infection stimulates the expression of MIF in the gastric inflammatory cells,which may play a significance role in gastric inflammation and ulceration.
-
Purpose: To observe the effects of electroacupuncture therapy on T cells and activity of NK cell in the patient of Chemotherapy. Method: Electro-acupuncture therapy was simultaneously applied during chemotherapy, T cells and activity of NK cell of patients were determined before electroacupuncture treatment (before chemotherapy) and after 4-course electro-acupuncture treatments. Results: Before chemotherapy, CD3 was low within the normal range, CD4 was much lower than the normal range, and CD8, CD4/CD8 and activity of NK cell were within the normal range. After one month of chemotherapy combined with electro-acupuncture, no decline of all the indices was found (P>0.05). Conclusion: Electro-acupuncture can really increase the immune function of patients of chemotherapy.The suppression of both immunity and hematopoiesis caused by chemotherapy is mainly characterized by declines of T cells subpopulations (CD3, CD4, CD8), activity of NK cell, WBC count and humoral immunity. Since 1996, the authors have applied electro-acupuncture therapy for 28 cases of malignant tumor in the process of chemotherapy with good results as reported in the following.
-
高效抗病毒治疗促使艾滋病患者免疫功能重建
The characteristics of AIDS is that HIV infection induces CD4 + T cell defects that includes a quantitave CD4 + T cells depletion and a loss of T helper cells function leading to a progressive immune deficiency. Early report indicated that this immune deficiency was irreversible, even with the antiretroviral therapies. More recently, however, clinical benifits of hightly active antiretroviral therapies (HAART) are increasingly evidenced by resolving opportunistic infections and malignancies, as well as declining hospitalization and mortality rates. This suggests that potent and sustained suppression of viral replication, at least to some extent, is associated with reconstitution of the immune system even in adult patients treated at advanced stages of the disease. Such immune reconstitution which was demonstrated firstly by a French reseach group includes (the group leader is Pr Brigitte Autran)(1) a rapid rise in CD4 + and CD8+ T cells followed by a slower CD4 + T cell increase; (2) a rapid rise in memory CD4 + T cells during the first three months of treatment by late increase in naive T cells coexpressing CD45RA and CD62L molecules after 3 months of efficient antiviral treatment;(3) a significant reduction of CD4 and CD8 activation markers in parallel to plasma virus load reduction;(4) a restoration in CD4 + T cell reactivity to recall antigens. These observations open new perspectives for the understanding of CD4 + T cells deficiency and therapeutic strategies of HIV infection. In the present review we will address some of the questions raised by immune restoration with HAART when administered at advanced stages of the disease.
-
一种新的效应CD4+T细胞亚群TH17的分化与功能
效应CD4+T细胞可以根据不同的分化途径和生物学功能分为不同的细胞亚群,包括TH1亚群、TH2亚群和调节性T细胞(regulatory T cells,子Treg)亚群.
-
Th1/Th2、Treg/Th17漂移在急性冠状动脉综合征中的研究进展
急性冠状动脉综合征(acute coronary syndrome,ACS)包括不稳定型心绞痛( unstable angina )和急性心肌梗死( acute myocardi-al infarction)。 ACS的病理生理学基础为在动脉粥样硬化的基础上粥样斑块破裂或裂隙。而动脉粥样硬化形成中存在着免疫的激活。研究[1]显示动脉粥样硬化斑块中存在着大量的免疫活性细胞---淋巴细胞和巨噬细胞,它们能启动特异及非特异免疫应答。另外,动脉粥样硬化斑块内还存在着免疫球蛋白、补体,还发现有补体的激活。这些提示免疫反应不仅与动脉粥样硬化斑块形成相关,也与斑块的易损性相关,这与ACS发病也有重要的关系。调节免疫对动脉粥样硬化的防治有着重要的意义,因此本文就辅助性T细胞( helper T cells ,Th)漂移与ACS的相关研究作一综述。
-
调节性T 细胞在糖尿病中的免疫保护作用及治疗进展
调节性T 细胞(regulatory T cells,Tregs)为一类特殊的T 淋 巴细胞亚群,因能够有效抑制自体免疫活性、预防人类的自体免 疫性疾病而受到广泛关注.Tregs 通过对其他免疫细胞的负调 节发挥维持免疫稳态的作用.已知Tregs 主要分为两类,天然的 Tregs(nTregs)以及外周抗原诱导的Tregs(iTregs).nTreg 是源自 胸腺的CD4 +CD25 +T 细胞,大约占外周CD4+T 细胞的5% ~ 10%[1] ,主要通过细胞间相互接触发挥免疫调节作用.iTregs 为 外周CD4 +CD25 -Treg 细胞由小剂量抗原或免疫抑制性细胞因 子诱导而成,是具有免疫活性的Foxp3+iTregs[2] ,包括Tr1、Th3 等细胞,主要通过分泌IL-10 和TGF-β发挥免疫负调控作用.
-
胃癌患者外周血CD4+CD25+Foxp3+调节性T细胞55例分析
临床上,胃癌是我国常见的腹部恶性肿瘤,已有不少研究显示,恶性肿瘤的发生发展与肿瘤患者的免疫功能密切相关.检测胃癌患者的免疫功能状态,对于探讨胃癌的发病机制(如免疫逃逸)和寻求新的治疗方案具有重要的临床价值.目前认为,T淋巴细胞在肿瘤患者的免疫系统中发挥着重要的作用,而CD4+辅助性T细胞是其中尤为重要的组成部分,包括以下4种类型的免疫细胞:Th1、Th2、Th17和调节性T细胞.近年来,发挥免疫耐受和免疫抑制功能的CD4 +CD25 +Foxp3+调节性T细胞群(regulatory T cells,Tregs)成为了肿瘤学者的热门对象[1-4],其中Foxp3是转录因子P亚家族的成员,其表达情况与CD4+CD25+Tregs发育和功能状态的关系十分密切[5].
-
CD+4CD+25CDlow/-127细胞体外抑制效应T淋巴细胞增殖的影响
1995年,Sakaguchi等 [1]率先在小鼠外周血中分离获得发挥免疫抑制功能的CD+4CD+25T淋巴细胞,被称为调节性T淋巴细胞(Regulatory T cells,Treg).Treg细胞不仅具有抑制自体反应性T淋巴细胞的功能,还能阻止效应性T淋巴细胞的过度增殖和活化,甚至能影响B淋巴细胞产生免疫球蛋白 [2].因此,对Treg细胞的功能研究正成为当今国内外的研究热点.本研究以CD+4CD+25CDlow-127为膜表面标志分选Treg细胞,利用羟基荧光素二醋酸盐琥珀酰亚胺脂(carboxyfluorescein diaeetate,succinimidyl ester,CFSE)方法 体外检测其对效应T淋巴细胞增殖的影响,从而为Treg细胞的功能研究奠定实验基础.
-
CO+4CD+25Clow/127-调节性T淋巴细胞与抗肿瘤免疫的研究进展
CO+4CD+25Clow127-调节性T淋巴细胞(regulatory T cells,Tregs)是维持机体免疫耐受的一种重要因素,也可通过相同的机制削弱机体的抗肿瘤效应.
-
维吾尔族宫颈癌患者外周血中CD+4CD+25CDlow/-127调节性T淋巴细胞与细胞因子的临床意义
宫颈癌是位居妇科肿瘤第一的恶性疾病[1].如何有效预防和治疗宫颈癌,提高患者生存质量是目前的研究热点.CD+4 CD+25 Treg在维持自身耐受和免疫稳定的同时,也抑制着免疫系统对肿瘤的免疫应答[2].经TGF-β和IL-10处理的细胞可获得明显的抑制效应性T淋巴细胞增殖的作用,炎症因子IFN-γ则与Treg起相反的作用[3].维吾尔族是宫颈癌高发民族,死亡率高[4].本研究通过流式细胞术检测维吾尔族宫颈癌患者外周血CD+4 CD+25 CDlow/-127 Treg细胞含量,采用ELISA检测血清中细胞因子TGF-β、IL-10及IFN-γ的水平,以便有针对性地开展新疆地区宫颈癌防治工作.
-
调节性T细胞与慢性感染性疾病
近年来,随着细胞及分子免疫学等相关学科的飞速发展,对T细胞及其亚群功能与作用机制的研究也日渐深入.近年来,调节性T细胞(regulatory T cells, Treg)的效应和意义受到了高度关注,它作为一个具有调节功能的成熟T细胞亚群,参与了自身免疫性疾病、移植耐受、肿瘤免疫、微生物感染等发病过程[1],本文拟重点介绍Treg在慢性感染性疾病中的作用及其机制的研究进展.
-
CD4+ CD25+ 调节T细胞对肝癌特异性细胞毒性T淋巴细胞杀伤活性的影响
CD4+ CD25+调节性T细胞(regulatory T cells,Treg)是体内主要的免疫调节细胞,研究发现多种肿瘤患者的外周血及肿瘤局部均有Treg的比例增高,提示Treg在肿瘤的发生发展过程中可能起重要的作用.
-
细胞因子白细胞介素-35研究进展
近年来,有关细胞因子研究的诸多进展对于人们深刻理解和认识机体免疫反应的生理功能与病理意义具有重要作用.2007年,Collison等[1]首次描述了白细胞介素-35(interleukin-35,IL-35)的组成及其功能,发现IL-35是小鼠体内专一由调节性T细胞(regulatory T cells,Treg)分泌,并对免疫功能发挥调控效应的重要细胞因子.新近的资料证实,IL-35参与了宿主免疫反应的调节过程,对其的研究日益受到高度关注.现就有关研究进展进行综述.
-
调节性T细胞在移植免疫中的辅助诊断价值
目前诊断移植后排斥反应的金标准是移植活检,但活检不仅有创而且在评估亚临应排斥反应方面存在局限性.
-
树突状细胞在免疫应答中的作用及其临床意义
树突状细胞(dendritic cell,DC)是指呈典型树突状或伪足状突起、膜表面高表达MHCⅡ类分子、能移行至次级淋巴器官和刺激初始型T细胞(naive T cells) 增殖活化,并具有一些相对特异性表面标志的专职抗原提呈细胞(professional antigen presenting cell,pAPC)[1].DC是目前已知的功能强抗原递呈细胞(antigen presenting cell,APC),参与抗原识别、加工处理和提呈,是机体免疫反应始动者,在免疫应答诱导中,具独特地位[2].DC与免疫激活和免疫耐受密切相关,在抗感染、肿瘤、移植排斥和自身免疫疾病等过程中发挥重要作用,是当今国内外免疫学界研究的热点.本文主要就DC的生物学特性、免疫应答中的作用及其临床意义综述如下.
-
去T细胞脐血低温保存的研究
目前的研究表明,非血缘关系的脐血移植(umbilical cord blood transplantation, CBT) 也是重要的造血干细胞来源[1].而脐血库的建立为开展配型不合的CBT提供了保证.但是,CBT可能发生移植物抗宿主病(graft versus host disease, GVHD),尤其是配型不合的CBT,从而影响移植成功率.免疫毒素(immunotoxin,IT)能有效去除脐血T细胞而对造血祖细胞无明显影响[2],为预防重度GVHD提供了有效手段.我们观察了IT去除脐血T细胞后的冻存效果.报告如下.
-
HIV感染后人体树突状细胞功能变化及其临床意义的研究进展
人体的树突状细胞(Dendritic cells,DC)是免疫系统中功能强的一种抗原提呈细胞.它不仅能够显著刺激纯真T细胞(Naive T cells)进行增殖,而且在先天性免疫和获得性免疫过程中均有重要作用[1].
-
T cell receptors (TCR) are a collection of several proteins located in the outer membrane of the T cell. Mature T cell has definitive TCR genes,which may be used as a clonal marker. To investigate whether TCR gene analysis may be useful for diagnosis of myasthenia gravis (MG) with thymoma, we used the cDNA of the β- subunit of the TCR served as probes to analyze the structure of the TCR β gene and to determine the extent of diversity between MG with thymoma and MG without thymoma. Eighteen patients were clinically diagnosed generalized MG, eight of them had thymoma. Peripheral blood lymphocytes (PBL) were obtained at or shortly before thymectomy (except one, 2 years after thymectomy). When DNA was digested with EcoRI and hybridized with the 32P labeled probes, two germline bands of 10.5kb and 4.0 kb appeared in DNAs from all 18 patients. The 10.5 kb band contains the C β 1 region and the 4.0kb fragment the C β 2 region, respectively. The 4.0 kb band is usually reduced as compared with the 10.5kb band. This can be explained by the preferential rearrangement of the C β 2 gene in T cell with MG. We were able to demonstrate a rearranged band of 9kb in EcoRI- digested DNAs of four patients. All the four patients had been found to have thymoma , of which 2 were lymphocyte and 2 mixed type. But rearranged bands could not be detected in the DNAs of another four patients with thymoma, of which 2 were epithelial and 2 mixed type. When DNA was digested with HindIII and BglII, germline bands appeared in DNAs from all patients. No rearranged bands could be observed . Single rearranged bands were detected in four patients with thymoma, this showed that specific TCR gene rearrangement may be related to certain types of thymoma . Detection of TCR gene rearrangement might be helpful to the early diagnosis of MG with thymoma . But we were unable to find gene rearrangement in another four thymoma (2 were epithelial and 2 mixed type). There are three possible explanations for the results. First, thymoma has heterogeneity. Second, TCR gene rearrangement only reflects the clonal origin of the T cell tumor. Third, in one patient, peripheral blood lymphocytes were obtained two years after thymectomy, neoplastic T cells maybe disappear. Thus, our results suggested that TCR gene rearrangement in patients with MG may be helpful for diagnosing certain type of thymoma.
-
NKT细胞与免疫耐受的研究进展
自然杀伤T细胞(nature killer T cells, NKT细胞)是一类新型的免疫调节细胞,不仅具有NK细胞的自然杀伤功能,也具有T细胞的免疫调节作用,因此已成为现代免疫学的研究的热点之一.尽管NKT细胞在体内数量相对较少,但种类复杂,功能多样.近年来研究发现,NKT细胞在免疫耐受中也扮演着重要的角色,如可以防止母体妊娠时对胎儿的免疫排斥、抑制自身免疫病的发生、介导器官移植免疫耐受的形成等.本文将对NKT细胞在免疫耐受中的作用的研究进展做一综述.
-
长期低水平苯接触对工人外周血调节性T细胞的影响
高浓度苯对血液系统和免疫系统具有明显的毒性作用[1-3].但长期低水平苯接触对工人免疫系统的影响还不清楚.有研究表明,职业苯接触工人CD8淋巴细胞亚型比例异常增多,而CD8淋巴细胞可能参与了骨髓造血细胞的免疫损伤.